Quasipucciniastrum agrimoniae, gen. et sp. nov. on Agrimonia (Rosaceae) from China

Figures & data

Table 1. Herbarium specimens used for molecular phylogenetic analyses.

				GenBank accession no. Collection
Species	Host	Locality and data of	Specimen no.^a	LSU	ITS
Cronartium coleosporioides	Castilleja linariaefolia	Montana USA, 1914	MICH253346	MK208284	MK193824
C. comandrae	Comandra umbellata	Wisconsin USA, 1955	MICH253364	MK208293	MK193825
C. comandrae	C. richardsiana	Canda	NY00267638	MK208294	MK193826
C. comandrae	C. pallida	USA	NY03106200	MK208295	MK193827
C. flaccidum	Paeonia lactiflora	Ussuriysk Russia, 2003	HMAS89229	MK208286	MK193819
C. flaccidum	P. lactiflora	Jilin China, 2003	HMAS89231	MK208289	MK193822
C. flaccidum	Pinus tabulaeformis	Inner Mongolia China, 1984	HMAS82784	MK208287	MK193818
C. flaccidum	P.taiwanensis	Henan China, 1981	HMAS44164	MK208288	MK193816
C. flaccidum	Quercus aquifolioides	Tibet China, 2010	HMAS242642	MK208290	MK193821
C. flaccidum	Q. aquifolioides	Tibet China, 2010	HMAS242641	MK208291	MK193820
C. quercuum	Q. glandulifera	Jiangxi China, 1996	HMAS82717	MK208292	MK193817
C. ribicola	Ribes nigrum	USA	NY00267051	MK208296	MK193828
C. ribicola	Pinus strobus	Ontario Canada, 1952	NY00267053	MK208298	MK193829
C. ribicola	Pedicularis yezoensis	Nagano Japan, 1990	HMAS66843	MK208297	MK193830
C. strobilinum	Pinus taeda	Florida USA, 1982	FLAS-F-53,222	MK208285	MK193823
Peridermium filamentosum	Pinus ponderosa	Arizona USA, 1957	MICH300081	MK208299	MK193831
Quasipucciniastrum agrimoniae	Agrimonia pilosa	Beijing China, 1998	HMAS82312	MK208264	MK193835
Q. agrimoniae	A. pilosa	Gansu China, 1992	HMAS67301	MK208261	MK193832
Q. agrimoniae	A. pilosa	Gansu China, 1992	HMAS67302	MK208273	MK193844
Q. agrimoniae	A. pilosa	Gansu China, 1992	HMAS67306	MK208262	MK193833
Q. agrimoniae	A. pilosa	Gansu China, 1992	HMAS67309	MK208263	MK193834
Q. agrimoniae	A. pilosa	Gansu China, 2003	HMAS134791	MK208268	MK193839
Q. agrimoniae	A. pilosa	Guangxi China, 1997	HMAS77430	MK208271	MK193842
Q. agrimoniae	A. pilosa	Guizhou China, 2015	HMAS248096	MK208283	MK193854
Q. agrimoniae	A. pilosa	Heilongjiang China, 2003	HMAS89584	MK208277	MK193848
Q. agrimoniae	A. pilosa	Heilongjiang China, 2004	HMAS136005	MK208274	MK193845
Q. agrimoniae	A. pilosa	Heilongjiang China, 2015	HMAS248094	MK208280	MK193851
Q. agrimoniae	A. pilosa	Heilongjiang China, 2015	HMAS248095	MK208281	MK193852
Q. agrimoniae	A. pilosa	Inner Mongolia China, 2000	HMAS172175	MK208272	MK193843
Q. agrimoniae	A. pilosa	Ningxia China, 2000	HMAS172172	MK208270	MK193841
Q. agrimoniae	A. pilosa	Ningxia China, 2000	HMAS172173	MK208265	MK193836
Q. agrimoniae	A. pilosa	Sichuan China, 2016	HMAS248093	MK208279	MK193850
Q. agrimoniae	A. pilosa	Sichuan China, 1989	HMAS63888	MK208266	MK193837
Q. agrimoniae	A. pilosa	Sichuan China, 1989	HMAS63892	MK208275	MK193846
Q. agrimoniae	A. pilosa	Sichuan China, 2010	HMAS243033	MK208276	MK193847
Q. agrimoniae	A. pilosa	Yunnan China, 2007	HMAS199430	MK208269	MK193840
Q. agrimoniae	A. pilosa	Yunnan China, 2011	HMAS248097	MK208282	MK193853
Q. agrimoniae	A. pilosa	Tibet China, 2011	HMAAS244481	MK208267	MK193838
Q. agrimoniae	A. pilosa	Yunnan China, 2016	HMAS248092	MK208278	MK193849

^aFLAS-F: University of Florida Herbarium, USA; HMAS: Fungarium, Chinese Academy of Sciences, China; MICH: University of Michigan Herbarium, USA; NY: New York Botanical Garden, USA.

Table 2. Sequence data retrieved from GenBank and used for phylogenetic analyses.

		GenBank accession no.
Species	Host plants	ITS	LSU	Reference
Chrysomyxa arctostaphyli	Arctostaphylos sp.	DQ200930.1	AY700192.1	Matheny et al. (2007)
C. chiogenis	Gaultheria hispidula	GU049452.1	GU049532.1	Feau et al. (2011)
C. ledi	Picea abies	HM037711.1	HM037707.1	Kaitera et al. (2010)
C. ledicola	P. mariana	GU049417.1	GU049520.1	Feau et al. (2011)
C. nagodhii	Rhododendron groenlandicum	GU049431.1	GU049524.1	Feau et al. (2011)
C. neoglandulosi	Ledum glandulosum	GU049498.1	GU049550.1	Feau et al. (2011)
C. piperiana	L. macrophyllum	GU049497.1	GU049565.1	Feau et al. (2011)
C. rhododendri	L. lapponicum	GU049467.1	GU049560.1	Feau et al. (2011)
C. rhododendri	Rhododendron ferrugineum	GU049471.1	GU049570.1	Feau et al. (2011)
C. vaccinii	Vaccinium parvifolium	GU049463.1	GU049561.1	Feau et al. (2011)
C. woroninii	Ledum groenlandicum	GU049462.1	GU049540.1	Feau et al. (2011)
Coleosporium. cacaliae	Adenostyles alliariae	KY810462.1	KY810462.1	Beenken et al. (2017)
Col. campanulae	Campanula rapunculoides	KY810465.1	KY810465.1	Beenken et al. (2017)
Col. euphrasiae	Rhinanthus alectorolophus	KY810469.1	KY810469.1	Beenken et al. (2017)
Col. inulae	Inula salicina	KY810470.1	KY810470.1	Beenken et al. (2017)
Col. ipomoeae	Ipomoea sp	MF769624.1	MF769639.1	McTaggart and Aime (2018)
Col. petasitidis	Petasites hybridus	KY810471.1	KY810471.1	Beenken et al. (2017)
Col. plumeriae	Plumeria sp	MF769629.1	GU145555.1	McTaggart and Aime (2018) Holcomb and Aime (2010)
Col. senecionis	Jacobaea alpina	KY810472.1	KY810472.1	Beenken et al. (2017)
Col. senecionis	Senecio ovatus	KY810473.1	KY810473.1	Beenken et al. (2017)
Col. solidaginis	Solidago virgaurea	KY810481.1	KY810481.1	Beenken et al. (2017)
Col. solidaginis	S. gigantea	KY810483.1	KY810483.1	Beenken et al. (2017)
Col. tussilaginis	Tussilago farfara	KY810485.1	KY810485.1	Beenken et al. (2017)
Cronartium conigenum	Pinus leiophylla	L76486.1	–	Vogler and Bruns (1998)
Cro. ribicola	–	DQ533975.1	AF522166.1	–
Endocronartium harknessii	Pinus sp.	DQ206982.1	AY700193.1	Matheny et al. (2007)
Hyalopsora polypodii	Cystopteris fragilis	–	AF426229.1	Maier et al. (2003)
H. polypodii	Deparia petersenii	–	KJ698627.1	–
Melampsorella caryophyllacearum	Caryophyllaceae Cerastium	–	MG907233.1	Aime et al. (2018)
M. caryophyllacearum	Abies alba	–	AF426232.1	Maier et al. (2003)
M. betulinum	Betula pubescens	KF031556.1	KF031539.1	McKenzie et al. (2013)
M. betulinum	Alnus cordata	KF031559.1	KF031544.1	McKenzie et al. (2013)
M. betulinum	Betula nana	KF031562.1	KF031549.1	McKenzie et al. (2013)
M. hiratsukanum	Alnus incana	KF031554.1	KF031541.1	McKenzie et al. (2013)
M. hiratsukanum	A. rhombifolia	KC313889.1	KC313888.1	Blomquist et al. (2014)
Milesina philippinensis	Nephrolepis sp.	–	KM249868.1	McTaggart et al. (2014)
Milesina sp.	Pinaceae Abies	–	MG907234.1	Aime et al. (2018)
M. vogesiaca	Polystichum aculeatum	–	MG907235.1	Aime et al. (2018)
Naohidemyces vaccinii	Vaccinium ovatum	–	DQ354563.1	Aime (2006)
N. vaccinii	Vaccinum	–	KJ698628.1	Padamsee et al. (2014)
Peridermium harknessii	Pinus contorta	L76506.1	–	Vogler and Bruns (1998)
Pileolaria terebinthi	Pistacia atlantica	HM639742.1	HM639742.1	Alaei et al. (2012)
P. terebinthi	P. atlantica	HM639743.1	HM639743.1	Alaei et al. (2012)
Pucciniastrum actinidiae	Actinidia arguta	AB221446.1	AB221403.1	Liang (2006)
P. actinidiae	A. rufa	AB221448.1	AB221405.1	Liang (2006)
P. boehmeriae	Boehmeria tricuspis	AB221450.1	AB221393.1	Liang (2006)
P. boehmeriae	B. platanifolia	AB221451.1	AB221391.1	Liang (2006)
P. corni	Cornus kuosa	AB221437.1	AB221409.1	Liang (2006)
P. corni	C. kuosa	AB221436.1	AB221408.1	Liang (2006)
P. fagi	Fagus crenata	AB221425.1	AB221378.1	Liang (2006)
P. fagi	F. crenata	AB221420.1	AB221374.1	Liang (2006)
P. fagi	F. crenata	AB221424.1	AB221375.1	Liang (2006)
P. fagi	F. japonica	AB221421.1	AB221376.1	Liang (2006)
P. fagi	F. crenata	AB221423.1	AB221377.1	Liang (2006)
P. hikosanense	Acer rufinerve	AB221441.1	AB221388.1	Liang (2006)
P. hikosanense	A. rufinerve	AB221440.1	AB221389.1	Liang (2006)
P. hydrangeae-petiolaris	Hydrangea petiolaris	AB221438.1	AB221384.1	Liang (2006)
P. hydrangeae-petiolaris	H. petiolaris	AB221439.1	AB221385.1	Liang (2006)
P. kusanoi	Clethra barbinervis	AB221429.1	AB221400.1	Liang (2006)
P. kusanoi	C. barbinervis	AB221426.1	AB221402.1	Liang (2006)
P. kusanoi	C.abarbinervis	AB221430.1	AB221401.1	Liang (2006)
P. kusanoi	C. barbinervis	AB221427.1	AB221399.1	Liang (2006)
P. kusanoi	Clethra barbinervis	AB221428.1	AB221398.1	Liang (2006)
P. miyabeanum	Viburnum furcatum	AB221443.1	AB221397.1	Liang (2006)
P. miyabeanum	V. furcatum	AB221442.1	AB221394.1	Liang (2006)
P. styracinum	Styrax japonica	AB221433.1	AB221417.1	Liang (2006)
P. styracinum	S. japonica	AB221432.1	AB221418.1	Liang (2006)
P. styracinum	S. japonica	AB221431.1	AB221416.1	Liang (2006)
P. tiliae	Tilia mandshurica	AB221455.1	AB221412.1	Liang (2006)
P. tiliae	T. japonica	AB221453.1	AB221414.1	Liang (2006)
P. tiliae	T. japonica	AB221454.1	AB221415.1	Liang (2006)
P. yoshinagai	Stewartia monadelphagene	AB221435.1	AB221410.1	Liang (2006)
P. yoshinagai	S. monadelphagene	AB221434.1	AB221411.1	Liang (2006)
Quasipucciniastrum agrimoniae	Agrimonia eupatoria	–	AF426234.1	Maier et al. (2003)
Q. agrimoniae	–	KJ486537.1	KJ725376.1	Yang (2015)
Q. agrimoniae	–	KJ486536.1	KJ725375.1	Yang (2015)
Q. agrimoniae	Agrimonia sp.	–	MG907236.1	Aime et al. (2018)
Thekopsora guttata	Galium odoratum	–	AF426231.1	Maier et al. (2003)
T. minima	Ericaceae Vaccinium	–	MG907243.1	Aime et al. (2018)
T. minima	Vaccinium corymbosum	–	KY991374.1	Shands et al. (Unpublished)
T. symphyti	Symphytum officinale	–	AF426230.1	Maier et al. (2003)
Uredinopsis osmundae	Dryopteridaceae Athyrium	–	MG907244.1	–
U. osmundae	Osmundaceae Osmunda	–	MG907245.1	–
U. pteridis	Pteridium esculentum	–	KM249869.1	McTaggart et al. (2014)
Uredinopsis sp.	–	–	AF522181.1	–

(–): No information from GenBank.

Figure 1. Bayesian 50% majority-rule consensus tree based on concatenated data of rDNA ITS and LSU sequences. Pileolaria terebinthi was used as outgroup. Values on the branches indicate maximum likelihood bootstrap values and Bayesian posterior probabilities. Hyphen indicates that bootstrap values were less than 75% and Bayesian posterior probabilities less than 0.80.

Figure 2. Morphological characters of Quasipucciniastrum agrimoniae. A: Uredinia (white arrow) on the hypophyllous leaf surface. B: No uredinia and telia on eiphyllous leaf surface. C: Uredinium with echinulate ostiolar cells (white arrow) observed by SEM. D: Urediniospores with echinulate spines observed by SEM. E: Uredinium with well-developed ostiolar cells (black arrow) observed by LM. F: Uredinium with peridium cell (black arrow). G: Urediniospores observed by LM. H: Vertical section of hypophyllous telia. I: Subepidermal teliospores with apparently thickened apical wall. J: Subglobose teliospores with vertical or oblique septa. Bars: A, B = 0.6 mm; C, E, G, H, I, J = 20µm; D, F = 10µm.

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