Elicitors and precursor induced effect on L-Dopa production in suspension cultures of Mucuna pruriens L.

Figures & data

Figure 1. Effectof methyl jasmonate on cell growth in suspension cultures of M. pruriens.

Figure 2. Effect of pectin on cell growth in suspension cultures of M. pruriens.

Figure 3. Effect of yeast extract on cell growth in suspension cultures of M. pruriens.

Figure 4. Effect of chitin on cell growth in suspension cultures of M. pruriens.

Figure 5. Effect of L-tyrosine on cell growth in suspension cultures of M. pruriens.

Table 1. Effect of elicitor methyl jasmonate on the production of L-Dopa^α in suspension cultures of Mucuna pruriens L.

Day	Control	Concentration of methyl jasmonate in μmol l^−1
		50	100	150	200
3	1.92 ± 0.04	2.60 ± 0.21	4.32 ± 0.02	5.93 ± 0.03	24.17 ± 0.12
6	2.18 ± 0.11	3.01 ± 0.03	5.61 ± 0.04	6.52 ± 0.05	31.92 ± 0.14
9	2.32 ± 0.12	7.89 ± 0.07	9.01 ± 0.03	12.12 ± 0.02	45.93 ± 0.32
12	2.35 ± 0.10	3.03 ± 0.02	6.04 ± 0.03	9.23 ± 09	24.13 ± 0.21
15	2.36 ± 0.12	3.03 ± 0.02	3.03 ± 0.02	3.03 ± 0.02	3.03 ± 0.02
Note: ^αData are expressed as an average of at least three separate experiments.

Table 2. Effect of elicitor chitin on the production of L-Dopa^α in suspension cultures of Mucuna pruriens L.

Day	Control	Concentration of chitin in mg l^−1
		50	100	150	200
3	1.92 ± 0.04	5.92 ± 0.02	6.52 ± 0.04	7.84 ± 0.07	12.02 ± 0.01
6	2.18 ± 0.11	9.23 ± 0.09	9.98 ± 0.09	11.13 ± 0.02	14.09 ± 0.12
9	2.32 ± 0.12	4.32 ± 0.02	5.80 ± 0.12	6.42 ± 0.05	6.32 ± 0.01
12	2.35 ± 0.10	3.23 ± 0.04	3.02 ± 0.01	4.46 ± 0.02	5.01 ± 0.32
15	2.36 ± 0.12	2.41 ± 0.34	2.41 ± 0.02	2.01 ± 0.32	2.05 ± 0.20
Note: ^αData are expressed as an average of at least three separate experiments.

Table 3. Effect of elicitor pectin on the production of L-Dopa^α in suspension cultures of Mucuna pruriens L.

Day	Control	Concentration of pectin in mg l^−1
		50	100	150	200
3	1.92 ± 0.04	12.02 ± 0.01	14.93 ± 0.12	19.97 ± 0.23	21.09 ± 0.12
6	2.18 ± 0.11	14.83 ± 0.32	19.57 ± 0.13	25.56 ± 0.23	35.35 ± 0.33
9	2.32 ± 0.12	17.05 ± 0.13	23.43 ± 0.34	31.23 ± 0.17	42.82 ± 0.31
12	2.35 ± 0.10	3.21 ± 0.01	3.21 ± 0.01	3.21 ± 0.01	4.33 ± 0.01
15	2.36 ± 0.12	2.54 ± 0.62	2.36 ± 0.02	2.41 ± 0.32	2.45 ± 0.32
Note: ^αData are expressed as an average of at least three separate experiments.

Table 4. Effect of elicitor yeast extract on the production of L-Dopa^α in suspension cultures of Mucuna pruriens L.

Day	Control	Concentration of yeast extract in mg l^−1
		50	100	150	200
3	1.92 ± 0.04	16.53 ± 0.35	23.67 ± 0.12	35.45 ± 0.31	42.78 ± 0.14
6	2.18 ± 0.11	25.76 ± 0.42	32.56 ± 0.32	45.93 ± 3.02	56.67 ± 4.12
9	2.32 ± 0.12	31.52 ± 0.12	42.79 ± 1.42	50.01 ± 4.32	62.35 ± 5.43
12	2.35 ± 0.10	12.73 ± 0.21	20.78 ± 0.52	26.84 ± 0.15	30.87 ± 0.28
15	2.36 ± 0.12	7.96 ± 0.23	8.74 ± 0.12	9.46 ± 0.46	9.46 ± 0.46
Note: ^αData are expressed as an average of at least three separate experiments.

Table 5. Effect of precurssor L-tyrosine on the production of L-Dopa^α in suspension cultures of Mucuna pruriens L.

Day	Control	Concentration of L-tyrosine in mM l^−1
		50	100	150	200
3	1.92 ± 0.04	10.52 ± 0.23	11.52 ± 0.41	27.75 ± 0.82	39.42 ± 1.32
6	2.18 ± 0.11	15.67 ± 0.21	23.53 ± 0.32	32.79 ± 0.65	50.98 ± 5.37
9	2.32 ± 0.12	20.46 ± 0.12	30.51 ± 0.78	42.92 ± 3.57	75.52 ± 6.13
12	2.35 ± 0.10	26.32 ± 0.42	45.23 ± 3.65	62.86 ± 5.23	89.91 ± 7.02
15	2.36 ± 0.12	30.47 ± 0.72	50.63 ± 2.41	74.09 ± 6.51	94.72 ± 7.06
Note: ^αData are expressed as an average of at least three separate experiments.