Chromosome and karyotype analysis of Hibiscus mutabilis f. mutabilis

Figures & data

Table 1. Root tip preprocessing schedule of Hibiscus mutabilis f. mutabilis seeds.

Processing method	Preprocessing time (h)
Saturated Santochlor	1	2	3	4
Ice/water mixture	12	24	36	48

Figure 1. Somatic cell mitotic metaphase chromosomal image of Hibiscus mutabilis f. mutabilis (×400).

Figure 2. Karyotype analysis of Hibiscus mutabilis f. mutabilis.

Figure 3. Chromosome karyotype patterning of Hibiscus mutabilis f. mutabilis.

Table 2. Chromosome relative length, arm ratio and type of Hibiscus mutabilis f. mutabilis.

No.	Relative length	Relative length coefficient	Chromosome length type	Arm ratio	Centromere position type
1	5.55 + 5.34 = 10.89	2.50	L	1.04	m
2	4.04 + 4.09 = 8.14	1.87	L	1.01	m
3	4.61 + 3.26 = 7.87	1.81	L	1.42	m
4	3.29 + 3.72 = 7.01	1.61	L	1.13	m
5	3.97 + 2.89 = 6.86	1.58	L	1.37	m
6	3.40 + 2.74 = 6.13	1.41	L	1.24	m
7	1.84 + 3.54 = 5.38	1.24	M2	1.92	sm
8	3.03 + 2.70 = 5.74	1.32	L	1.12	m
9	2.62 + 2.86 = 5.48	1.26	L	1.09	m
10	2.67 + 2.75 = 5.45	1.25	M2	1.04	m
11	2.67 + 2.75 = 5.42	1.25	M2	1.03	m
12	1.94 + 2.59 = 4.54	1.04	M2	1.33	m
13	1.92 + 2.42 = 4.34	1.00	M1	1.26	m
14	2.38 + 2.12 = 4.50	1.04	M2	1.13	m
15	2.49 + 2.04 = 4.53	1.04	M2	1.22	m
16	1.63 + 2.42 = 4.05	0.93	M1	1.48	m
17	2.2 + 1.94 = 4.15	0.95	M1	1.13	m
18	2.15 + 1.91 = 4.06	0.93	M1	1.12	m
19	1.97 + 1.99 = 3.96	0.91	M1	1.01	m
20	2.15 + 1.87 = 4.02	0.92	M1	1.15	m
21	2.23 + 1.79 = 4.02	0.92	M1	1.25	m
22	2.02 + 1.88 = 3.90	0.90	M1	1.07	m
23	2.15 + 1.80 = 3.95	0.91	M1	1.19	m
24	1.74 + 2.02 = 3.76	0.86	M1	1.17	m
25	1.94 + 1.88 = 3.83	0.88	M1	1.03	m
26	1.79 + 1.96 = 3.75	0.86	M1	1.10	m
27	1.74 + 1.99 = 3.73	0.86	M1	1.15	m
28	2.13 + 1.72 = 3.85	0.88	M1	1.23	m
29	2.28 + 1.60 = 3.88	0.89	M1	1.43	m
30	1.19 + 2.23 = 3.42	0.79	M1	1.87	sm
31	0.96 + 2.36 = 3.31	0.76	M1	2.46	sm
32	2.23 + 1.57 = 3.79	0.87	M1	1.42	m
33	2.23 + 1.53 = 3.76	0.86	M1	1.45	m
34	1.89 + 1.74 = 3.63	0.83	M1	1.09	m
35	1.45 + 1.94 = 3.39	0.78	M1	1.34	m
36	1.79 + 1.72 = 3.51	0.81	M1	1.04	m
37	1.68 + 1.79 = 3.47	0.80	M1	1.06	m
38	1.63 + 1.79 = 3.42	0.79	M1	1.09	m
39	1.53 + 1.83 = 3.36	0.77	M1	1.20	m
40	1.68 + 1.72 = 3.40	0.78	M1	1.02	m
41	1.63 + 1.72 = 3.35	0.77	M1	1.06	m
42	1.79 + 1.61 = 3.40	0.78	M1	1.11	m
43	1.61 + 1.53 = 3.14	0.72	S	1.05	m
44	0.8 + 0.82 = 1.62	0.37	S	1.03	m
45	0.8 + 0.76 = 1.56	0.36	S	1.06	m
46	0.54 + 0.70 = 1.24	0.29	S	1.28	m

Note: ^aL = long; M₁= slightly short; M₂ = slightly long; S = short; m = metacentric; sm = submetacentric.