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Primary Research

Effect of honey on purified equine myeloperoxidase activity and superoxide radical production in activated Polymorphonuclear neutrophils

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Pages 379-386 | Received 17 Nov 2014, Accepted 20 Apr 2015, Published online: 08 Jun 2015

Figures & data

Table 1. Total polyphenol and flavonoid contents of the three honey samples.

Figure 1. Effect of various concentrations of honey on lucigenin-dependent chemiluminescence. The percentage of inhibition of the chemiluminescence of each honey sample was compared to the control (CTRL), taken as 100% (assay performed without honey). Chemiluminescence values were obtained for unstimulated polymorphonuclear neutrophils in phosphate-buffered saline (PBS) or with the addition of dimethylsulfoxide (DMSO; vehicle of phorbol 12-myristate 13-acetate). Data are shown as mean ± SD (n = 9). ***p < 0.0001 for all honey samples compared to control.

Figure 1. Effect of various concentrations of honey on lucigenin-dependent chemiluminescence. The percentage of inhibition of the chemiluminescence of each honey sample was compared to the control (CTRL), taken as 100% (assay performed without honey). Chemiluminescence values were obtained for unstimulated polymorphonuclear neutrophils in phosphate-buffered saline (PBS) or with the addition of dimethylsulfoxide (DMSO; vehicle of phorbol 12-myristate 13-acetate). Data are shown as mean ± SD (n = 9). ***p < 0.0001 for all honey samples compared to control.

Figure 2. Interaction of honey with myeloperoxidase (MPO) studied by the specific immunological extraction followed by enzymatic detection (SIEFED) technique. Control (CTRL): activity of the enzyme in the absence of honey, taken as 100%. Data are shown as mean ± SD (n = 12). ***p < 0.0001 for all samples and at all concentrations versus CTRL, except for LH3 at 2% concentration; ns = not significant vs control.

Figure 2. Interaction of honey with myeloperoxidase (MPO) studied by the specific immunological extraction followed by enzymatic detection (SIEFED) technique. Control (CTRL): activity of the enzyme in the absence of honey, taken as 100%. Data are shown as mean ± SD (n = 12). ***p < 0.0001 for all samples and at all concentrations versus CTRL, except for LH3 at 2% concentration; ns = not significant vs control.

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