Figures & data
Figure 1. Effect of Solanum melongena extracts on nitric oxide (NO) production: (a) ethanol extract; (b) hexane extract; (c) ethyl acetate extract; (d) water extract. RAW 264.7 cells were stimulated with 10 µg/ml lipopolysaccharide (LPS) alone or in the presence of the indicated concentrations of extract, and the amount of nitrite was evaluated after 24 h. Results are presented as mean ± SD from three independent experiments with three replicates each. *Significantly different value from negative control at 95% confidence level; **significantly different from negative control at 99% confidence level. L-NAME = N-Nitro-L-arginine methyl ester hydrochloride (positive control).
Figure 2. Effects of Solanum macrocarpon extracts on nitric oxide (NO) production: (a) ethanol extract; (b) hexane extract; (c) ethyl acetate extract; (d) water extract. RAW 264.7 cells were stimulated with 10 µg/ml lipopolysaccharide (LPS) alone or in the presence of indicated concentrations of extract and the amount of nitrite was evaluated after 24 h. Results are presented as mean ± SD from three independent experiments with three replicates each. *Significantly different value from negative control at 95% confidence level; **significantly different from negative control at 99% confidence level. L-NAME = N-Nitro-L-arginine methyl ester hydrochloride (positive control).
Table 1. Median inhibitory concentration (IC50) (inhibition of nitric oxide production) of various extracts of Solanum melongena and Solanum macrocarpon.
Table 2. Viability of RAW 264.7 cells treated with Solanum melongena and Solanum macrocarpon extracts at 200 µg/ml.
Figure 3. Immunoblot analysis of the effects of various extracts of Solanum melongena extracts on inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells: (a) ethanol extract; (b) hexane extract; (c) ethyl acetate extract; (d) water extract. Results are mean ± SD of three independent experiments with three replicates each. *p < 0.05 and **p < 0.01 indicate significant differences from LPS-induced cells. Dexa = dexamethasone (positive control).
Figure 4. Immunoblot analysis of the effects of various extracts of Solanum macrocarpon extracts on inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)stimulated RAW 264.7 cells: (a) ethanol extract; (b) hexane extract; (c) ethyl acetate extract; (d) water extract. Results are mean ± SD of three independent experiments with three replicates each. *p < 0.05 and **p < 0.01 indicate significant differences from LPS-induced cells. Dexa = dexamethasone (positive control).
