Exploring diet-dependent shifts in methanogen and methanotroph diversity in the rumen of Mehsani buffalo by a metagenomics approach

Figures & data

Table 1. Shotgun sequencing data summary for M50GL and M100GL.

	Green roughage
	M50GL	M100GL
N (number of replicates)	4	4
Uploaded sequence count	14,72,376	17,74,141
Post QC sequence count	14,13,707	15,95,926
Mean sequence length (bp)	177	200
Annotated functional categories	95,471	1,30,587
α-Diversity (species)	295	211

Figure 1. Rarefaction curve for M50GL and M100GL groups by MEGAN. x-Axis = percentage of reads; y-axis = number of leaves in taxonomy.

Figure 2. Heatmap showing the taxonomic classification of the M50GL and M100GL groups.

Figure 3. Extended error bar chart showing comparative abundance between M50GL and M100GL for different groups of Proteobacteria.

Figure 4. Tree showing species-level classification of phylum Euryarcheota for the M50GL and M100GL groups.

Figure 5. KEGG analysis showing enzymes involved in the methane metabolism pathway. The bar chart represents the abundance of enzymes for both M50GL and M100GL groups, where blue represents the M50GL group and red represents the M100GL group. x-Axis = abundance of enzymes; y-axis = E.C. number of enzymes involved in methane metabolism.