Morphological characters and genetic relationship between catechins-rich and -poor tea tree (Camellia sinensis L.) lines

Figures & data

Table 1. Catechins content of selected tea trees used in this study.

Catechins-rich and -poor lines	Individual line	Catechins content (mg g^−1 dry weight)
		EC	ECG	EGCG	EGC	Total catechins
Catechins-rich lines	HR-52	35.73	0.26	2.6	83.43	122.02
	HR-29	44.32	0.17	4.06	185.88	234.43
	HR-82	48.32	0.22	0.65	83.69	132.88
	HR-123	32.11	0.13	1.59	82.63	116.46
	HR-55	5.64	0.24	1.98	108.18	116.04
Catechins-poor lines	HP-19	12.75	0.09	0.55	41.16	54.55
	HP-108	11.19	0.09	0.13	40.68	52.09
	HP-130	7.23	0.11	0.02	37.9	45.26
	HP-150	16.52	0.21	0.04	49.75	66.52
	HP-18	14.99	0.22	0.03	70.1	85.34
Note: The content of catechin extracts were measured with HPLC System (Gilson, USA) equipped with a Tosoh TSK ODS-80Ts (4.6 × 250 mm, Tosoh) column, acetonitrile: 0.2% phosphoric acid (H3PO4) in dH2O (25: 75, v/v) with a flow rate of 0.5 ml min^−1, and UV detector at 280 nm.

Figure 1. A typical leaf of Camellia sinensis L.

Table 2. Morphology analysis from selected tea tree lines.

	Tea tree lines	Shoot length (mm)	Leaf width (mm)	Leaf length (mm)	Leaf area (cm^2)
High producing lines	HR-29	61.6 ± 11.7	17.1 ± 3.6	51.8 ± 9.7	6.4 ± 2.3
	HR-52	59.9 ± 0.2	16.3 ± 4.6	45.8 ± 10.6	5.5 ± 2.7
	HR-55	89.4 ± 4.8	20.4 ± 2.1	52.8 ± 6.8	7.6 ± 1.6
	HR-82	70.7 ± 5.3	19.7 ± 4.3	44.4 ± 8.8	6.4 ± 2.3
	HR-123	88.5 ± 8.6	20 ± 5.4	53.1 ± 14.7	7.9 ± 4.2
	Average	74 ± 14	18.7 ± 1.9	49.6 ± 4.1	6.8 ± 0.9
Low producing lines	HP-18	61.6 ± 11.5	16.4 ± 4.3	38.8 ± 10.0	4.4 ± 2.1
	HP-19	64.4 ± 6.8	22.4 ± 5.1	53.2 ± 11.2	8.7 ± 3.7
	HP-108	52.1 ± 13.6	18.3 ± 3.7	53.3 ± 7.4	6.9 ± 2.3
	HP-138	55.4 ± 8.2	24.2 ± 5.3	55.7 ± 12.0	9.8 ± 3.6
	HP-150	30.7 ± 8.5	16.4 ± 4.1	42.2 ± 11.5	5.1 ± 2.5
	Average	52.8 ± 13.3	19.5 ± 5.6	48.6 ± 7.6	7 ± 2.3

Figure 2. Morphology analysis from selected tea tree lines. (a) The average shoot length from selected tea tree lines. (b) The cluster analysis based on morphology analysis from selected tea tree lines. 1: HR-52, 2: HR-29, 3: HR-82, 4: HR-123, 5: HR-55, 6: HP-19, 7: HP-108, 8: HP-138, 9: HP-150 and 10: HP-18. This cluster analysis based on each variable was done by using the SPSS 17.0 statistics program.

Figure 3. The cluster analysis based on four variables (leaf width, leaf length, leaf area, and shoot length) from selected tea tree lines. 1: HR-52, 2: HR-29, 3: HR-82, 4: HR-123, 5: HR-55, 6: HP-19, 7: HP-108, 8: HP-138, 9: HP-150, and 10: HP-18. This cluster analysis based on each variable was done by using the SPSS 17.0 statistics program.

Table 3. The community similarity among the four major stands within selected tea tree lines.

	A	B	C	D	EC	EGCG
A
B	0.684*
C	0.947**	0.873**
D	0.313	0.461	0.412
EC	−0.370	−0.193	−0.324	0.043
EGCG	−0.278	0.250	−0.074	0.369	0.562*
ECG	−0.505	−0.614	−0.627	0.002	0.184	0.197
Notes: A: Shoot length, B: Leaf width, C: Leaf length, and D: Leaf area.
**: P < 0.01, *: P < 0.05.

Table 4. RAPD primers and their sequences used to detect genetic diversity.

Primer	Nucleotide sequence (5′ to 3′)	No. of amplified DNA bands	No. of polymorphic bands
OPB10	CGTCTGGGAC	41	3
OPB12	CCTTGACGCA	34	3
OPB16	TTTGCCCGGA	47	7
OPB17	AGGGACGAG	35	4
OPB18	CCACAGCAGT	57	5
OPB19	ACCCCCGAAG	36	3
OPC4	CCGCATCTAC	24	5
OPC5	GATGACCGCC	30	4
OPC6	GAACGGACTC	29	4
OPC7	GTCCCGACGA	34	3
OPC8	TGGACCGGTG	20	0
OPC9	CTCACCGTCC	27	5
OPC10	TGTCTGGGTG	44	6
OPC11	AAAGCTGCGG	32	5
OPC12	TGTCATCCCC	20	4
OPC13	AAGCCTCGTC	23	6
OPC15	GACGGATCAG	37	5
OPC16	CACACTCCAG	46	5
OPC17	TTCCCCCCAG	34	5
OPC20	ACTTCGCCAC	24	6
OPD1	ACCGCGAAGG	27	7
OPD2	GGACCCAACC	21	2
Total		722	97

Figure 4. Polymorphisms of selected tea tree lines by RAPD analysis using various primers. OPB18 (a); OPC4 (b); OPC7 (c); and OPC10 (d).

Figure 5. A UPGMA phenogram of catechins-rich and -poor tea tree lines based on RAPD analysis. 1: HR-52, 2: HR-29, 3: HR-82, 4: HR-123, 5: HR-55, 6: HP-19, 7: HP-108, 8: HP-138, 9: HP-150, and 10: HP-18.