In vitro selection of salt-tolerant Ailanthus altissimaSwingle

Figures & data

Figure 1. Growth rate of protoplast-derived A. altissima callus.

Table 1. Growth and survival rate of salt-selected A. altissima callus.

[NaCl] (%) suspension medium	[NaCl] (%) solid medium	Fresh weight (g)*	Callus survival rate (%)**
				Control	0	5.0	100
0.1	2.5	77
0.5	3.0	55
1.0	N.E.	N.E.
1.5	N.E.	N.E.
2.0	N.E.	N.E.
0.1	0	4.5	100
	0.1	2.9	77
	0.5	2.6	90
	1.0	2.5	90
	1.5	N.E.	N.E.
	2.0	N.E.	N.E.
0.5	0	4.2	90
	0.1	2.9	77
	0.5	2.4	66
	1.0	2.1	55
	1.5	2.0	55
	2.0	N.E.	N.E.
1.0	0	3.3	55
	0.1	2.6	33
	0.5	2.9	55
	1.0	2.4	33
	1.5	2.0	11
	2.0	1.7	11
1.5	0	3.8	33
	0.1	3.4	22
	0.5	3.4	33
	1.0	2.9	11
	1.5	2.8	11
	2.0	1.0	11
2.0	0	2.8	11
	0.1	2.5	11
	0.5	N.E.	N.E.
	1.0	2.5	11
	1.5	N.E.	N.E.
	2.0	N.E.	N.E.
*Fresh weight of cells was measured on solid medium with 4.5 μM 2,4-D, 0.5 μM BA and 30 g^− 1 sucrose.
**Percentage of survival rate of callus of A. altissima measured after 3 weeks in culture.
N.E., not estimated.

Figure 2. Salt tolerance of A. altissima callus.

Figure 3. Growth rate of salt-tolerant A. altissima callus.

Figure 4. Proline content of salt-resistant A. altissima callus.

Figure 5. Organogenesis in salt-tolerant A. altissima callus line. A: further growth of shoot primordial. B: development of a micro-shoot.

Table 2. Regeneration of salt-tolerant A. altissima callus.

[NaCl] (%)	0	0.1	0.5	1.0	1.5	2.0
No. of shoots	5.7 ± 1.5*	4.0 ± 1.0	1.7 ± 0.8	−**	−	−
*Values represents the standard deviation.
**No response.