Figures & data
Figure 1. Visfatin expression in mouse brown and white adipocytes and stromal vascular fractions. mRNA expression determined by RT-qPCR in Stroma Vascular Fraction (SVF) and adipocyte fraction (AF) from scWAT, eWAT and iBAT of C57BL/6 mice. Perilipin-1 was used as control of adipocyte purification, and leptin as a white adipocyte marker. Histograms represent mean ± sem of 8 mice. *: p<0 .05.
Figure 2. Visfatin expression in mouse brown and white adipocytes. (A) Cells from SVF of scWAT, eWAT and iBAT depots were differentiated in white or brown adipocytes and used for mRNA level evaluation by RT-qPCR. Perilipin-1 was used as control of adipogenesis, and leptin as white adipocyte marker. (B) Intracellular and extracellular visfatin protein levels were quantified by EIA in SVF-derived white and brown adipocytes after 16 h of incubation in absence or presence of 16 μM CL316,243. Histograms represent mean ± sem of 3 independent experiments (B). *: p<0 .05.
Figure 3. Visfatin is preferentially expressed in mouse BAT, but is not correlated to brown adipocyte recruitment/activation. mRNA expression was determined by RT-qPCR in iBAT, scWAT and eWAT from C57BL/6 mice exposed (4 days) at a temperature of 4±2°C (A) or treated with CL316,243 for 1 week (B). Control animals were respectively maintained at housing temperature (21±2°C) or injected with the vehicle (NaCl 0.9%, w/v). Perilipin-1 was used as a control marker of adipose tissue. Histograms represent mean ± sem of 8 mice. *: p<0 .05.
Figure 4. Visfatin is preferentially expressed in rat iBAT, but is not induced with BAT activation. mRNA expression was determined by RT-qPCR in iBAT and eWAT from Wistar rats exposed overnight at a temperature of 4±2°C (A) or treated with CL316,243 for 1 week (B). Control animals were maintained near to rat thermoneutral temperature (23±2°C) or injected with the vehicle (NaCl 0.9%, w/v). Perilipin-1 was used as a control marker of adipose tissue. Histograms represent mean ± sem of 4 rats. *: p<0 .05.
Figure 5. Visfatin expression and secretion in human white and brown adipocytes. (A) mRNA levels were evaluated by qRT-PCR in matched biopsies of scWAT and BAT from 6 healthy human adult patients. mRNA expression determined in human subcutaneous adipose tissue SVF-derived white and brown adipocytes (B) or hMADS adipocytes (C). Perilipin-1 was used as control of adipogenesis and leptin as white adipogenic marker. (D) Intracellular and extracellular visfatin EIA quantification in hMADS white and brown adipocytes after 16 h of incubation with or without 1μM CL316,243. Histograms represent mean ± sem of 6 tissue samples (A) or 3 independent experiments (B, C, D). *: p<0 .05.
Table 1. Sequence of primers used for gene expression analysis.