Unspecific DNA recombination in AdipoqCre-ER^T2 – mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent

Figures & data

Figure 1. Inducible TDTO Expression Induction of TDTO expression in AT explants derived from young, heterozygous, male Adipoq-CreERT²xTDTO reporter mice (b, c) and non-treated controls (a). TDTO fluorescence was detected before and 72 h after TAM induction. Scale bar represents 250 µm

Figure 2. Non-induced TDTO expression in AT from Adipoq-CreER^T2xTDTO mice Non-induced TDTO expression is studied in Adipoq-CreER^T2xTDTO reporter mice of different sex, genotype and age. A, Bar graph summarizes non-stimulated TDTO expression in young (<15 weeks) Adipoq-CreER^T2xTDTO mice of different sex and genotype. B, Linear regression is shown for non-induced TDTO expression in correlation to age of male and female mice. The red line represents the overall regression independent of sex. C and d, representative images for homozygous and heterozygous, female and male mice (c) and for heterozygous, female mice of different ages (d). TDTO fluorescence red, Lipidtox^TM fluorescence blue. Data are presented as means ± SEM. Scale bar 250 µm. * p < 0.05 and **p < 0.01