Figures & data
Table 1. Primer sets
Table 2. Distribution of number, age and weight of lambs in each treatment group
Figure 1. Messenger RNA concentrations (fg mRNA per 50 ng total RNA) for Uncoupling Protein 1 (UCP1) (a) and leptin (b) in perirenal fat from 14-day old control (CONT) lambs gestated and delivered under normoxic conditions, and 14-day old lambs exposed to long term moderate gestational hypoxia in utero (LTH) then maintained post-birth at either high altitude (HA) or near sea level (SL) through 14 days’ post-birth. Lambs exposed to LTH in utero exhibited significantly decreased UCP-1 (a, p < 0.05) in both LTH-HA and LTH-SL lambs. LTH-SL lambs (but not LTH-HA lambs) had elevated mRNA concentration of leptin (b; p < 0.05) compared to normoxic control lambs
Figure 2. Messenger RNA concentrations (fg mRNA per 50 ng total RNA) and protein levels (Densiometric Units; D.U.) for Adiponectin (a, b) and DIO2 (c, d) in perirenal fat from 14-day old control (CONT) lambs gestated and delivered under normoxic conditions, and 14-day old lambs exposed to long term moderate gestational hypoxia in utero (LTH) then maintained post-birth at either high altitude (HA) or near sea level (SL) through 14 days post-birth. Lambs exposed to LTH in utero had reduced mRNA and protein compared to CONT lambs in the PRF. However, for mRNA, only the LTH-SL lambs did this reach significance. For both LTH cohorts, however, protein was significantly reduced. For DIO2, both LTH-SL and LTH-HA groups exhibited significantly reduced mRNA, while for protein, a reduction was only noted in the LTH-HA group. (lanes 1–3 CONT; Lanes 4–6 LTH-SL; Lanes 7–9 LTH-HA)
Figure 3. Messenger RNA concentrations (fg mRNA per 50 ng total RNA) and protein levels (Densiometric Units; D.U.) for PPARγ (a, b) and PGC1α (c, d). LTH-SL lambs (but not LTH-HA lambs) had elevated mRNA concentration of PPARγ (a; p < 0.05). A similar pattern was observed for protein (b). Lambs exposed to LTH in utero exhibited significantly decreased mRNA PGC1α (c, p < 0.001) in both LTH-HA and LTH-SL lamb compared to controls. Despite a similar pattern in the LTH groups, only the LTH-HA lambs exhibited a significant decrease in protein compared to controls. (d; p < 0.05; lanes 1–3 CONT; Lanes 4–6 LTH-SL; Lanes 7–9 LTH-HA)
Figure 4. Messenger RNA concentrations (fg mRNA per 50 ng total RNA) for PRDM16 (a), RIP140 (b), PPARα (c), HSD11B1 (d), HSD11B2 (e), FGFR1 (f), and βKlotho (g) in perirenal fat from 14-day old control (CONT) lambs gestated and delivered under normoxic conditions and 14-day old lambs exposed to long term moderate gestational hypoxia in utero (LTH) then maintained post-birth at either high altitude (HA) or near sealevel (SL) through 14 days post-birth. Differences were noted in mRNA for only PPARα, and that was restricted to the LTH-SL cohort (c)
