Circulating microRNA changes in patients with impaired glucose regulation

Figures & data

Table 1. Anthropometric measurements and clinical variables of IGR participants before and after the intervention

	Before (Mean ± SD)	After (Mean ± SD)		p Value *≤0.025
All IGR participants (n = 20)
Age (y)	60.85 (12.1)
Weight (kg)	101.5 (23.9)		99.2 (24.7)	0.025*
Height (cm)	168.85 (10.8)
BMI	35.66 (7.6)		34.86 (7.9)	0.023*
Waist-hip	0.953 (0.08)		0.950 (0.07)	0.763
HDL (mmol/L)	1.37 (0.71)		1.30 (0.38)	0.685
LDL (mmol/L)	2.75 (1.01)		2.66 (1.04)	0.593
Triglycerides (mmol/L)	1.5 (0.79)		1.62 (0.70)	0.464
Insulin (pmol/L)	190.80 (259.9)		270.93 (588.4)	0.482
Total cholesterol	4.69 (1.20)		4.83 (1.11)	0.328
HOMA-S	68.4 (42.59)		74.64 (56.86)	0.757
HOMA-B	134.35 (62.24)		113.93 (39.22)	0.078
FPG	5.58 (0.87)		5.90 (1.25)	0.149
HbA1 c	44.55 (2.52)		45.11 (10.99)	0.820
Patients maintained or lost 3% or more in weight (n = 17)
Weight (kg)	100.6 (24.5)		97.2 (24.8)	0.001*
BMI	35.5 (7.48)		34.2 (7.71)	0.001*
Waist-hip	0.95 (0.09)		0.95 (0.07)	0.726
HDL (mmol/L)	1.36 (0.77)		1.28 (0.40)	0.691
LDL (mmol/L)	2.75 (1.09)		2.52 (1.12)	0.232
Triglycerides (mmol/L)	1.55 (0.85)		1.62 (0.76)	0.697
Insulin (pmol/L)	207.84 (279.20)		301.55 (635.87)	0.487
Total cholesterol	4.69 (1.28)		4.74 (1.18)	0.741
HOMA-S	73.28 (44.79)		75.84 (60.47)	0.913
HOMA-B	126.35 (51.71)		111.87 (36.41)	0.236
FPG	5.50 (0.87)		5.28 (0.43)	0.369
HbA1 c	43.57 (1.39)		39.42 (1.51)	0.006*

*p values refer to paired T-test analysis of measures at baseline and after the lifestyle intervention. BMI: body mass index; HDL: high-density lipoprotein cholesterol; LDL: low-density lipoprotein cholesterol; HOMA-S: Homoeostasis Model Assessment insulin sensitivity; HOMA-B: Homoeostasis Model Assessment beta-cell function; FPG: fasting plasma glucose; HbA1c: glycated haemoglobin.

Figure 1. (a) MicroRNAs expression in serum of IGR individuals (n = 20) at baseline and 6 months after the lifestyle intervention. Data were analysed by Paired T-test and are expressed as Mean ± SD (p ≤ 0.025). (b) MicroRNAs expression in serum of IGR individuals which maintained weight or lost 3% of weight only (n = 17) at baseline and 6 months after the lifestyle intervention. Data were analysed by Paired T-test and are expressed as Mean ± SD (p ≤ 0.025)

Figure 2. (a) Correlation matrix among microRNAs and clinical variables in all IGR individuals at baseline. Analysis were done by Pearson’s correlation, p ≤ 0.05. The r values for each correlation are shown in the squares. (b) Correlation matrix among microRNAs and clinical variables in all IGR individuals post-intervention. Analysis were done by Pearson’s correlation, p ≤ 0.05. The r values for each correlation are shown in the squares

Figure 3. (a) Correlation matrix among microRNAs and clinical variables in IGR individuals who maintained or lost 3% in weight or more at baseline. Analysis were done by Pearson’s correlation, p ≤ 0.05. The r values for each correlation are shown in the squares. (b) Correlation matrix among microRNAs and clinical variables in IGR individuals who maintained or lost 3% in weight or more post-intervention. Analysis were done by Pearson’s correlation, p ≤ 0.05. The r values for each correlation are shown in the squares

Figure 4. (a) Scatter plots representing the correlations among microRNAs and target genes in all IGR individuals at baseline. Analysis were done by Pearson’s correlation, p ≤ 0.05, r ≥ 0.350. (b) Scatter plots representing the correlations among microRNAs and target genes in all IGR individuals post-intervention. Analysis were done by Pearson’s correlation, p ≤ 0.05 r ≥ 0.350

Data availability statement

Data available on request due to privacy/ethical restrictions