Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion

Figures & data

Table 1. Primer sequence.

	Forward Primer (5′-3′)	Reverse Primer (5′-3′)
Pref-1	CCTGCAGCACACCCAGG	GGTCTCGCACTTGTTGAGGA
C/EBP β	GGGAATCTTTTCCGTTTCAAGCA	TGCCCCCAAAAGGCTTTGTA
C/EBP α	TATAGGCTGGGCTTCCCCTT	AGCTTTCTGGTGTGACTCGG
PPAR γ	CCAGAAGCCTGCATTTCTGC	CACGGAGCTGATCCCAAAGT
SLC2A4	GTTCTTTCATCTTCGCCGCC	TTCCCCATCTTCGGAGCCTA
GAPDH	ACATCGCTCAGACACCATG	TGTAGTTGAGGTCAATGAAGGG

Figure 1. Adipocytes promote platinum drug resistance in OvCa cells.

(a-b) The effects of Adipo-CM treatment on cell viability and apoptosis level were detected by CCK-8 and flow cytometry. (c) The expression of proliferation-related and apoptosis-related proteins was detected by western blot. (d) The IC₅₀ values of different concentrations of cisplatin and carboplatin treatment on OvCa cells were detected by CCK-8. *p<0.05.

Figure 2. Adipocytes promote OvCa resistance to platinum drugs through paracrine secretion of IGF1.

(a) The expression of IGF1 in HPAd cells was detected by western blot. (b) The concentration of IGF1 in the culture media of HPAd cells was detected by ELISA. (c-d) The effects of different treatments on SKOV3 cell viability and apoptosis level were detected by CCK-8 and flow cytometry. (e) The expression of proliferation-related and apoptosis-related proteins was detected by western blot. (f) The IC₅₀ values of different concentrations of cisplatin and carboplatin treatment on SKOV3 cells were detected by CCK-8. *VS oe-NC+DMSO, #VS oe-IGF1+DMSO, *p<0.05.

Figure 3. OJS inhibits adipocyte paracrine secretion of IGF1 to regulate OvCa progression.

(a-b) The expression of IGF1 in HPAd cells and cell culture supernatant was detected by western blot and ELISA, respectively. (c-d) The effects of different treatments on SKOV3 cell viability and apoptosis level were detected by CCK-8 and flow cytometry. (e) The expression of proliferation-related and apoptosis-related proteins was detected by western blot. (f) The IC₅₀ values of different concentrations of cisplatin and carboplatin treatment on SKOV3 cells were detected by CCK-8. *VS Adipo-CM, #VS Adipo-CM2, p<0.05.

Figure 4. OJS enhances the anticancer activity of cisplatin in SKOV3 and HPAd xenograft models.

(a-c) Tumour growth under different treatments. (d) The effects of OJS and cisplatin on the damage and morphological changes of major organs in mice were detected by H&E staining. (e) The expression of proliferation-related and apoptosis-related proteins was detected by IHC. *VS control, #VS cisplatin, p<0.05.

Data availability statement

The data that support the findings of this study are openly available in zenodo at https://doi.org/10.5281/zenodo.8035464, reference number 8,035,464.