Figures & data
Figure 1. Flow diagram of study population in the nurses’ health study and the health professionals follow-up study
![Figure 1. Flow diagram of study population in the nurses’ health study and the health professionals follow-up study](/cms/asset/3ece26bb-41c5-4f61-83d6-5fc6b87533db/koni_a_1956173_f0001_b.gif)
Figure 2. Tumor CD274 and PTEN expression in colorectal cancer. Tumor CD274 expression was evaluated based on immunostaining in the cytoplasm and membrane of tumor cells. Tumor CD274 expression was interpreted as negative (a), low (b), or high (c) according to membranous and cytoplasmic intensity. Tumor PTEN expression was evaluated based on immunostaining in the cytoplasm and nuclear of tumor cells. Cytoplasmic and nuclear PTEN expression level was classified as lost (d) or intact (e) according to cytoplasmic and nuclear intensity. Scale bars represent 50 μm
![Figure 2. Tumor CD274 and PTEN expression in colorectal cancer. Tumor CD274 expression was evaluated based on immunostaining in the cytoplasm and membrane of tumor cells. Tumor CD274 expression was interpreted as negative (a), low (b), or high (c) according to membranous and cytoplasmic intensity. Tumor PTEN expression was evaluated based on immunostaining in the cytoplasm and nuclear of tumor cells. Cytoplasmic and nuclear PTEN expression level was classified as lost (d) or intact (e) according to cytoplasmic and nuclear intensity. Scale bars represent 50 μm](/cms/asset/2f28f9eb-08e9-4224-af1e-cb86a6c0d243/koni_a_1956173_f0002_oc.jpg)
Table 1. Clinical, pathological, and molecular characteristics of colorectal cancer cases according to PIK3CA mutation and PTEN expression in tumor tissue
Table 2. Inverse probability weighting-adjusted ordinal logistic regression analysis to assess the association of tumor PIK3CA mutation or PTEN expression (predictor) with CD274 (PD-L1) expression (outcome)
Table 3. CD274 (PD-L1) expression score according to PIK3CA mutation and PTEN expression in tumor tissue
Table 4. Inverse probability weighting-adjusted ordinal logistic regression analysis to assess the association of the combination of PIK3CA mutation and PTEN expression (predictor) with CD274 (PD-L1) expression (outcome)
Supplemental Material
Download ()Availability of data and materials
The datasets generated and/or analyzed in this study are not publicly available. Further information including the procedures to obtain and access data from the Nurses’ Health Studies and the Health Professionals
Follow-up Study are described at https://www.nurseshealthstudy.org/researchers/ and