Genetic stability of vaccine strains by multilocus sequence typing and pulsed-field gel electrophoresis analysis: Implications for quality control of the leptospiral vaccine

Figures & data

Table 1. Information for 7 leptospiral vaccine strains used in this study

Species	Serogroup	Serovar	Strain	Host of Isolation	Source of Isolation	Year of Isolation
L. interrogans	Icterohaemorragiae	lai	Lai	Human	Sichuan, China	1958
L. interrogans	Autumnalis	autumnalis	Lin 4	Human	Zhejiang, China	1954
L. interrogans	Pomona	pomona	Luo	Human	Fujian, China	1958
L. interrogans	Grippotyphosa	linhai	Lin 6	Human	Zhejiang, China	1954
L. interrogans	Australis	australis	Wo 34	Human	China	1950s
L. interrogans	Hebdomadis	hebdomadis	Guang 229	Human	Guangdong, China	1950s
L. interrogans	Canicola	canicola	611	Human	China	1950s

Figure 1. Selected PFGE patterns and MLST types from 7 leptospiral vaccine strains during in vivo and in vitro passages. The unweighted pair group method with arithmetic averages (UPGMA) was used as the clustering method for PFGE profiles. Number of passages: −1 represented vaccine strains before inoculation in animals; 1, 10 and 20 represented the first, tenth, and twentieth passage of each strain in culture, respectively.