Figures & data
Figure 1. Schematic showing FUS-induced BBB opening with its potential effect in CNS immune modulation and immunotherapy.
Figure 2. Glial Fibrillary Acidic Protein (GFAP) immunofluorescence, neuronal nuclei (NeuN), and HE staining in the contralateral (A, B, C) and in the FUS-BBB opened brain (D, E, F). Neuron distributions (nuclei stained by NeuN; B and E) appeared similar on both sides of the brain, but concentrations of activated glial cells (stained by GFAP; A and D) were increased in the experimental lateral brain. HE-staining (C and F) showed that the tissue structure was not severely damaged by FUS treatment. Bar = 200 μm.
Figure 3. Representative fluorescent IHC analysis shows lymphocyte infiltration in the brain tumor and the corresponding lymphocyte population changes (particularly Treg and CTL populations). (A) Untreated brain tumor; (B) brain tumor treated with FUS-induced BBB opening; (C) brain tumor treated by IP IL-12 administration; (D) combined FUS with IL-12 administration, showing the enriched CTL infiltration and CTL/Treg ratio increase. Green: CD4+CD25+ lymphocytes (Treg); Red: CD3+CD8+ lymphocytes (CTL); Blue: DAPI-stained cell nucleus. Bar = 50 μm.
