Figures & data
Figure 1. Vaccination protocol and challenge calendar. Mice are immunized three times following a prime/boost scheme (days 0, 21 and 42) either with recombinant proteins or protein complexes, either with a plasmid coding for a protein of interest in the case of DNA immunization. Two weeks after the third immunization, mice are challenged (day 57) with M. abscessus, either by an aerosol way, either intravenously (IV). Lungs are collected, homogenized by dislocation and CFUs are counted at various times after bacterial challenge according to the type of challenge practised: day 1, 7, 14 and 21 for aerosol challenge or day 1, 15 and 45 for IV challenge.
