Figures & data
Table 1. Clinical trials and human laboratory studies of biologics for SUD.Footnote*
Figure 1. The frequency of hapten-specific B cells in blood predicts vaccine efficacy. Pre- and post-vaccination 6OXY-specific B cells were analyzed in 0.2 ml of blood collected before and after immunization with either 6OXY-KLH or KLH. Balb/c mice were immunized on days 0, 14 and 28, and challenged with 2.25 mg/kg oxycodone a week after the third immunization to measure the effect of immunization on oxycodone distribution. A) Immunization reduced distribution of oxycodone to the brain. Data are mean ± SEM. B) Early serum IgG antibody titers correlated to subsequent blockage of oxycodone distribution to the brain. C) The frequency of 6OXY-specific IgMhigh B cells prior to immunization correlated with vaccine efficacy in the 6OXY-KLH group. D) Increased frequency of 6OXY-specific IgMhigh B cells 14 days after the first immunization correlated to greater vaccine efficacy on oxycodone distribution to the brain. Frequencies are the % of total lymphocytes in the bound fraction after positive enrichment of blood. Data include 3 independent experiments with a total of n = 12 mice each group. *** p < 0.001 compared to KLH control. Copyright 2015. The American Association of Immunologists, Inc.
