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Human Vaccines & Immunotherapeutics Volume 13, 2017 - Issue 10
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Research Paper

HLA-DQB1*06 and breadth of Nef core region-specific T-cell response are associated with slow disease progression in antiretroviral therapy-naive Chinese HIV-1 subtype B patients

Weihua LiYouAn Hospital, Capital Medical University, Beijing, China;Beijing Liver Disease Research Institute, Beijing, China
,
Chuanyun LiYouAn Hospital, Capital Medical University, Beijing, China
,
Wei XiaYouAn Hospital, Capital Medical University, Beijing, China
&
Xiuhui LiYouAn Hospital, Capital Medical University, Beijing, ChinaCorrespondence[email protected]
Pages 2341-2347 | Received 02 Mar 2017, Accepted 06 Jun 2017, Published online: 18 Oct 2017

Figures & data

Figure 1. Magnitude of human immunodeficiency virus (HIV)-1 subtype B Nef core specific T cell responses in 29 HIV-1B-infected individuals. Measured as interferon (IFN)-γ enzyme-linked immunospot (ELISPOT), represented as spot-forming units (SFU)/106 peripheral blood mononuclear cells.

Figure 1. Magnitude of human immunodeficiency virus (HIV)-1 subtype B Nef core specific T cell responses in 29 HIV-1B-infected individuals. Measured as interferon (IFN)-γ enzyme-linked immunospot (ELISPOT), represented as spot-forming units (SFU)/106 peripheral blood mononuclear cells.

Figure 2. Magnitude and frequency of 10 HIV-1 subtype B Nef core OLPs. The total magnitude of T cell responses specific for each tested peptide is shown as bars in the left part of the graph. The frequency of each tested peptide is shown as bars on the right part of the graph.

Figure 2. Magnitude and frequency of 10 HIV-1 subtype B Nef core OLPs. The total magnitude of T cell responses specific for each tested peptide is shown as bars in the left part of the graph. The frequency of each tested peptide is shown as bars on the right part of the graph.

Figure 3. The heatmap for all patients and all peptides provide full picture with the ELISPOT responses. The right ordinate is the patient NO., The bottom abscissa is the peptide NO. The heatmap should define the cut-off as the detection limit.

Figure 3. The heatmap for all patients and all peptides provide full picture with the ELISPOT responses. The right ordinate is the patient NO., The bottom abscissa is the peptide NO. The heatmap should define the cut-off as the detection limit.

Figure 4. ELISPOT magnitude is associated with CD4+T cell count and viral load. The associations between ELISPOT magnitude (total spot forming units per 1 Million PBMCs and CD4+T cell count or viral load) were analyzed by Spearman's correlation. The Nef core-specific response was compared with CD4+T cell count (a) and viral load (b). P value is corrected by FDR.

Figure 4. ELISPOT magnitude is associated with CD4+T cell count and viral load. The associations between ELISPOT magnitude (total spot forming units per 1 Million PBMCs and CD4+T cell count or viral load) were analyzed by Spearman's correlation. The Nef core-specific response was compared with CD4+T cell count (a) and viral load (b). P value is corrected by FDR.

Figure 5. ELISPOT breadth is associated with CD4+T cell countand viral load. The associations between ELISPOT breadth [the number of reacting OLPs] and CD4+T cellcount (a) or viral load (b) were analyzed using the Kruskal-Wallis test. (n) Refers to the number of individuals. P value is corrected by FDR.

Figure 5. ELISPOT breadth is associated with CD4+T cell countand viral load. The associations between ELISPOT breadth [the number of reacting OLPs] and CD4+T cellcount (a) or viral load (b) were analyzed using the Kruskal-Wallis test. (n) Refers to the number of individuals. P value is corrected by FDR.

Figure 6. Comparison of CD4 count, VL, Magnitude and Breadth between DQB1*06 positive and negative group. P value is corrected by FDR.

Figure 6. Comparison of CD4 count, VL, Magnitude and Breadth between DQB1*06 positive and negative group. P value is corrected by FDR.

Figure 7. DQB1*06 allele-specific enzyme-linked immunospot (ELISPOT) magnitude were compared with viral load (VL) and CD4+ T cell count. P value is corrected by FDR.

Figure 7. DQB1*06 allele-specific enzyme-linked immunospot (ELISPOT) magnitude were compared with viral load (VL) and CD4+ T cell count. P value is corrected by FDR.

Table 1. Characteristics of the study patient cohort.

Table 2. Nef Core OLPs sequence, magnitude and breath.

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