Analysis of the dose-sparing effect of adjuvanted Sabin-inactivated poliovirus vaccine (sIPV)

Figures & data

Table 1a. Analyzed seroconversion of trivalent sIPV with different dose and adjuvanted group after the first immunization.

	Type I				Type II				Type III
Groups	R(%)	P1	P2		R(%)	P1	P2		R(%)	P1	P2
Full-dose sIPV	100	—	0.001		50	—	0.001		60	—	NS
1/4-dose sIPV	20	0.001	—		0	0.001	—		20	NS	—
1/4-dose+AS03	100	NS	0.001		100	NS	0.000		100	0.011	0.001
1/4-dose+BW006	100	NS	0.001		50	NS	0.033		60	NS	NS
1/4-dose+Poly:IC	70	NS	NS		0	0.001	NS		0	NS	NS
1/4 dose+AS03+ BW006	100	NS	0.001		100	NS	0.000		100	0.011	0.001
Total		P = 0.011				P = 0.000				P = 0.000

Note: Seroconversion refers to a reciprocal antibody titer of 8 or higher. Data are proportion(%) of subjects with seroconversion(n = 10).

p1 values are compared with the full-dose group, p2 values are compared with the 1/4-dose group. NS denotes not significant.

Table 1b. Analyzed seroconversion of trivalent sIPV with different dose and adjuvanted group after second immunization.

	Total Fisher's Exact Tests Value	Total p-value
Type I	5.609	0.153
Type II	7.194	0.129
Type III	4.987	0.416

Note: Seroconversion refers to a reciprocal antibody titer of 8 or higher. Data are proportion(%) of subjects with seroconversion(n = 10).

Table 2a. The geometric mean titers(GMTs) against Sabin strains of poliovirus after first immunization.

	GMT(95%CI)
Groups	Type I	Type II	Type III
Full-dose sIPV	22.90(9.50, 55.16)	3.78(0.31, 46.41)	3.57(0.38, 33.67)
1/4-dose sIPV	1.89(0.32, 11.03)	1.00(1.00, 1.00)	1.52(0.48, 4,81)
1/4-dose+AS03	57.04(18.21, 178.65)	67.08(11.09, 405.88)	163.27(87.88, 303.25)
1/4-dose+BW006	75.27(29.03, 195.12)	3.48(0.35, 35.06)	11.45(0.56, 232.81)
1/4-dose+Poly:IC	39.19(9.21, 166.80)	1.00(1.00, 1.00)	1.00(1.00, 1.00)
1/4 dose+AS03+ BW006	262.12(192.84, 356.29)	210.38(58.82, 752.66)	163.27(52.71, 505.59)
Total	52.26(30.13, 90.68)	14.49(6.46, 32.54)	20.87(9.68, 45.00)

Note: Full-dose referred to type = 30 DU, type II = 32 DU, type III = 45 DU. 1/4-dose referred to type I = 7.5 DU, type II = 8 DU, type III = 11.25 DU

Antibody titer of the first immunization after 28 days was expressed as geometric mean titers (GMT).

Table 2b. The geometric mean titers(GMTs) against Sabin strains of poliovirus after second immunization.

	GMT(95%CI)
Groups	Type I	Type II	Type III
Full-dose sIPV	145.51(34.25, 614.19)	46.88(6.90, 318.57)	215.44(49.39, 939.72)
1/4-dose sIPV	57.04(13.08, 248.83)	15.10(2.07, 110.10)	19.93(1.09, 365.51)
1/4-dose+AS03	524.20(165.20, 2046.44)	247.46(41.61, 1471.63)	882.27(600.34, 1296.28)
1/4-dose+BW006	262.12(150.07, 457.72)	57.04(2.47, 1317.35)	203.38(58.09, 382.30)
1/4-dose+Poly:IC	313.55(83.18, 1181.95)	43.37(3.76, 500.26)	110.84(25.44, 482.95)
1/4 dose+AS03+ BW006	1500.38(500.50,4497.80)	1073.49(462.06,2493.45)	2097.01(1060.72,4145.72)
Total	348.10(224.44, 527.47)	159.22(81.10, 312.61)	420.15(224.08, 787.59)

Note: Full-dose sIPV referred to type I = 30 DU, type II = 32 DU, type III = 45 DU. 1/4-dose referred to type I = 7.5 DU, type II = 8 DU, type III = 11.25 DU

Antibody titer of the second immunization after 28 days was expressed as geometric mean titers (GMT).

Figure 1a. Poliovirus antigen specific IgG after the first immunization.

*, **, ***, and **** refer to p<0.05, p<0.01, p<0.001, and p< 0.0001, respectively.

Figure 1b. Poliovirus antigen specific IgG after the second immunization.

*, **, ***, and **** refer to p<0.05, p<0.01, p<0.001, and p< 0.0001, respectively.

Figure 2. The weight of rats 3 days after the first immunization and every subsequent 7 days.

Figure 3a. IgG1 and IgG2a for type II poliovirus after the first immunization.

Note: Error bars are for a 95% upper CI.

Figure 3b. IgG1 and IgG2a for type II poliovirus after the second immunization.

Note: Error bars are for a 95% upper CI.