Neisserial PorB immune enhancing activity and use as a vaccine adjuvant

Figures & data

Figure 1. Electron micrograph of PorB proteosomes.

Micellar multi-molecular organized clusters of PorB termed “proteosomes”. The scale bar is in the lower left corner and measures 100nm. Each proteosome is approximately 10nm in diameter. This electron micrograph was generated by Dr Lee Wetzler

Figure 2. Effects of PorB as an adjuvant during vaccination.

(1) PorB recognition by TLR1/2 on antigen presenting cells (APCs) at the site of injection leads to TLR2 and MyD88-dependent signaling for increased costimulatory molecule expression and secretion of Th1 and Th2 cytokines as shown by Platt et al²² and Mosaheb et al.²³ (2) Enhanced APC activation promotes trafficking of APC-antigen to secondary lymphoid organs (SLOs) and (3) antigen uptake and processing for classical and cross-presentation inducing activation of antigen-specific CD4⁺ (as shown in green) and CD8⁺ T cells (as shown in blue).²⁴ (4) PorB enhances germinal center (GC) formation and production of both Th1 and Th2 IgGs.^23,24Follicular dendritic cell (FDC); CD4⁺ T follicular helper cells (Tfh); Memory B cell (MBC); Antibody secreting cell (ASC)

Platt A, MacLeod H, Massari P, Liu X, Wetzler L. In vivo and in vitro characterization of the immune stimulating activity of the neisserial porin PorB. Das G, ed. PLoS One. 2013;8(12):e82171. doi:10.1371/journal.pone.0082171.

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Mosaheb MM, Reiser ML, Wetzler LM. Toll-like receptor ligand-based vaccine adjuvants require intact MyD88 signaling in antigen-presenting cells for germinal center formation and antibody production. Front Immunol. 2017;8:225. doi:10.3389/fimmu.2017.00225.

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Reiser ML, Mosaheb MM, Lisk C, Platt A, Wetzler LM. The TLR2 binding neisserial porin PorB enhances antigen presenting cell trafficking and cross-presentation. Sci Rep. 2017. doi:10.1038/s41598-017-00555-4.

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