Study of the association of seventeen single nucleotide polymorphisms and their haplotypes in the TNF-α, IL-2, IL-4 and IL-10 genes with the antibody response to inactivated Japanese encephalitis vaccine

Figures & data

Table 1. Demographic characteristics of the IJEV NAbs SP and SN group

	Seropositive group (n = 160)	Seronegative group (n = 209)	P value
Ages (years)	8.099 ± 2.53	7.62 ± 2.439	.066
Sex (M/F)	74/86	107/102	.346
Antibody level
1:10	86
1:20	31
1:40	20
1:80	17
>1:80	6

Table 2. Allelic and genotypic distribution of 17 SNPs in the TNF-α, IL-2, IL-4 and IL-10 genes in the IJEV NAbs SP and SN group

Genes	SNPs	Allele/ Genotype		Seropositive group n (Fre.)	Seronegative group n (Fre.)	P value	X^2	OR
TNF-α	rs1800629	Allele	A	33(0.103)	48(0.115)	.614	0.254	0.886[0.554 ~ 1.417]
			G	287(0.897)	370(0.885)
		Genotype	AA	1(0.004)	0(0.000)	.376	1.954
			AG	31(0.194)	48(0.230)
			GG	128(0.800)	161(0.770)
	rs3093668	Allele	C	16(0.050)	15(0.036)	.343	0.897	1.414[0.688 ~ 2.905]
			G	304(0.950)	403(0.964)
		Genotype	CG	16(0.100)	15(0.072)	.333	0.938	1.437[0.688 ~ 3.002]
			GG	144(0.900)	194(0.928)
	rs3093726	Allele	C	8(0.025)	6(0.014)	.293	1.104	1.761[0.605 ~ 5.126]
			T	312(0.975)	412(0.986)
		Genotype	CT	8(0.050)	6(0.029)	.289	1.126	1.781[0.605 ~ 5.239]
			TT	152(0.950)	203(0.971)
IL-2	rs11932411	Allele	T	320(1.000)	418(1.000)
		Genotype	TT	160(1.000)	209(1.000)
	rs11575812	Allele	A	293(0.916)	387(0.926)	.609	0.261	0.869[0.508 ~ 1.488]
			G	27(0.084)	31(0.074)
		Genotype	AA	134(0.838)	178(0.852)	.505	1.365
			AG	25(0.156)	31(0.148)
			GG	1(0.006)	0(0.000)
	rs2069763	Allele	G	155(0.484)	199(0.476)	.823	0.050	1.034[0.772 ~ 1.384]
			T	165(0.516)	219(0.524)
		Genotype	GG	39(0.244)	46(0.220)	.815	0.408
			GT	77(0.481)	107(0.512)
			TT	44(0.275)	56(0.268)
	rs2069762	Allele	A	208(0.650)	269(0.644)	.856	0.033	1.029[0.759 ~ 1.395]
			C	112(0.350)	149(0.356)
		Genotype	AA	67(0.419)	86(0.411)	.982	0.036
			AC	74(0.463)	97(0.464)
			CC	19(0.119)	26(0.124)
	rs4833248	Allele	A	113(0.353)	150(0.359)	.872	0.026	0.975[0.720 ~ 1.322]
			G	207(0.647)	268(0.641)
		Genotype	AA	20(0.125)	27(0.129)	.987	0.026
			AG	73(0.456)	96(0.459)
			GG	67(0.419)	86(0.411)
IL-4	rs2243247	Allele	G	320(1.000)	418(1.000)
		Genotype	GG	160(1.000)	209(1.000)
	rs2243248	Allele	G	27(0.084)	34(0.081)	.882	0.022	1.041[0.614 ~ 1.764]
			T	293(0.916)	384(0.919)
		Genotype	GG	1(0.006)	2(0.010)	.891	0.231
			GT	25(0.156)	30(0.144)
			TT	134(0.838)	177(0.847)
	rs2243250	Allele	C	84(0.263)	123(0.294)	.341	0.906	0.854[0.616 ~ 1.183]
			T	236(0.738)	295(0.706)
		Genotype	CC	11(0.069)	16(0.077)	.569	1.126
			CT	62(0.388)	91(0.435)
			TT	87(0.544)	102(0.488)
	rs2070874	Allele	C	83(0.259)	122(0.292)	.329	0.954	0.850[0.613 ~ 1.178]
			T	237(0.741)	296(0.708)
		Genotype	CC	10(0.062)	15(0.072)	.569	1.129
			CT	63(0.394)	92(0.440)
			TT	87(0.544)	102(0.488)
	rs2227284	Allele	G	53(0.166)	85(0.203)	.193	1.697	0.778[0.532 ~ 1.136]
			T	267(0.834)	333(0.797)
		Genotype	GG	5(0.031)	7(0.033)	.328	2.229
			GT	43(0.269)	71(0.340)
			TT	112(0.700)	131(0.627)
	rs2243291	Allele	C	238(0.744)	297(0.711)	.317	1.003	1.182[0.852 ~ 1.642]
			G	82(0.256)	121(0.289)
		Genotype	CC	88(0.550)	102(0.488)	.493	1.416
			CG	62(0.388)	93(0.445)
			GG	10(0.062)	14(0.067)
	rs2243292	Allele	T	320(1.000)	418(1.000)
		Genotype	TT	160(1.000)	209(1.000)
IL-10	rs1800872	Allele	G	124(0.388)	152(0.364)	.507	0.441	1.107[0.820 ~ 1.495]
			T	196(0.613)	266(0.636)
		Genotype	GG	23(0.144)	30(0.144)	.620	0.957
			GT	78(0.487)	92(0.440)
			TT	59(0.369)	87(0.416)
	rs1800871	Allele	A	196(0.613)	266(0.636)	.507	0.441	0.903[0.669 ~ 1.220]
			G	124(0.388)	152(0.364)
		Genotype	AA	59(0.369)	87(0.416)	.620	0.957
			AG	78(0.487)	92(0.440)
			GG	23(0.144)	30(0.144)
	rs1800896	Allele	C	42(0.131)	47(0.112)	.437	0.605	1.193[0.765 ~ 1.860]
			T	278(0.869)	371(0.888)
		Genotype	CC	2(0.013)	2(0.010)	.730	0.630
			CT	38(0.237)	43(0.206)
			TT	120(0.750)	164(0.785)

Table 3. Allelic and genotypic distribution of the rs1800896 in IL-10 and rs1800629 in TNF-α gene in the IJEV NAbs SP and SN groups in females

	SNP			Seropositive group (n = 86)	Seronegative group (n = 102)	P value	X^2	OR
IL-10	rs1800896	Allele	C	27(0.157)	20(0.098)	.085	2.964	1.713[0.924 ~ 3.178]
			T	145(0.843)	184(0.902)
		Genotype	CC	2(0.023)	1(0.010)	.227	2.964
			CT	23(0.267)	18(0.176)
TNF-α	rs1800629	Allele	A	11(0.064)	23(0.113)	.376	0.784	1.320[0.713 ~ 2.443]
			G	161(0.936)	181(0.887)
		Genotype	AG	11(0.128)	23(0.225)	.083	2.999	0.504[0.230 ~ 1.104]
			GG	75(0.872)	79(0.775)

Table 4. Inheritance model analysis of the rs2243291 in IL-4 gene in the IJEV NAbs SP and SN group in males

Model	Genotype	Seropositive group (n = 74)	Seronegative group (n = 107)	OR (95% CI)	P value	AIC	BIC
Codominant	C/C	46 (62.2%)	52 (48.6%)	1	.130	246.9	256.5
	C/G	23 (31.1%)	49 (45.8%)	1.88 (1.00–3.56)
	G/G	5 (6.8%)	6 (5.6%)	1.06 (0.30–3.71)
Dominant	C/C	46 (62.2%)	52 (48.6%)	1	.071	245.6	252
	C/G-G/G	28 (37.8%)	55 (51.4%)	1.74 (0.95–3.18)
Recessive	C/C-C/G	69 (93.2%)	101 (94.4%)	1	.750	248.8	255.2
	G/G	5 (6.8%)	6 (5.6%)	0.82 (0.24–2.79)
Overdominant	C/C-G/G	51 (68.9%)	58 (54.2%)	1	.045	244.9	251.3
	C/G	23 (31.1%)	49 (45.8%)	1.87 (1.01–3.49)
Log-additive	–	–	–	1.41 (0.85–2.32)	.170	247.0	253.4

Table 5. Inheritance model analysis of the rs1800896 in IL-10 gene in the IJEV NAbs SP and SN group in females

Model	Genotype	Seropositive group (n = 86)	Seronegative group (n = 102)	OR (95% CI)	P value	AIC	BIC
Codominant	T/T	61 (70.9%)	83 (81.4%)	1	.230	262.3	272.0
	C/T	23 (26.7%)	18 (17.6%)	0.58 (0.29–1.16)
	C/C	2 (2.3%)	1 (1%)	0.37 (0.03–4.15)
Dominant	T/T	61 (70.9%)	83 (81.4%)	1	.092	260.4	266.9
	C/T-C/C	25 (29.1%)	19 (18.6%)	0.56 (0.28–1.10)
Recessive	T/T-C/T	84 (97.7%)	101 (99%)	1	.460	262.7	269.2
	C/C	2 (2.3%)	1 (1%)	0.42 (0.04–4.67)
Overdominant	T/T-C/C	63 (73.3%)	84 (82.3%)	1	.130	261.0	267.5
	C/T	23 (26.7%)	18 (17.6%)	0.59 (0.29–1.18)
Log-additive	–	–	–	0.58 (0.31–1.09)	.086	260.3	266.8