Quantitative characterization of the T cell receptor repertoires of human immunized by rabies virus vaccine

Figures & data

Table 1. Basic sequencing data for 4 volunteers pre-vaccination and post-vaccination

Sample	Status	Total Reads	Clonotype (reads)	Clonotype Percent (%)	Unique V family	Unique J family	Unique VJ pair	Unique CDR3aa sequence number	Unique CDR3nt sequence number
S1	pre-vaccination	18042152	8091394	44.84716679	45	14	457	193127	210807
S1	post-vaccination	20872265	9505175	45.53973898	45	14	462	286076	317454
S2	pre-vaccination	19321494	8855845	45.83416272	42	13	418	139310	150147
S2	post-vaccination	16895509	7765863	45.96406655	46	14	442	373561	418357
S3	pre-vaccination	19024646	8404508	44.17694815	45	14	491	198838	215431
S3	post-vaccination	18661470	8623195	46.20855163	45	14	486	502378	568806
S4	pre-vaccination	21524023	9652271	44.84417713	44	14	481	188998	203271
S4	post-vaccination	17834948	8177721	45.85222788	46	14	473	419599	468227

Figure 1. The diversity of repertoires of TCRBV before and after immunization with RABV vaccines in 4 volunteers. The volunteers were injected with anti-RABV vaccines for three times and TCR repertoires were detected by high throughput sequencing. The comparison of most frequent TCRBV before and after RABV vaccination was shown

Figure 2. Unique clonotypes in different degree of expansion in 4 volunteers before and after RABV vaccination. The clonotypes before and after vaccination were calculated and divided into five levels according to their frequencies

Figure 3. TCRBV diversity in 4 volunteers before and after RABV vaccination. The TCRBV diversity were evaluated by Shannon method

Figure 4. The usage frequencies of TRBV and TRBJ gene segments in 4 volunteers before and after RABV vaccination. (a) Frequency of TRBV segments before and after RABV vaccination. (b) Frequency of TRBJ segments before and after RABV vaccination

Figure 5. The usage frequencies of all possible distinct functional V-J pairing in 4 volunteers before and after RABV vaccination. The x axis represents all functional V genes and the y axis represents all functional J genes. The height of the bars is proportional to the frequency of a V-J pairing

Table 2. The overlap in the repertoires of TCRB CDR3 before and after immunization with RABV vaccines in 4 volunteers

Red boxes represent the overlap unique clonotypes between pre- and post- vaccination in the same volunteer. Green boxes represent the overlap unique clonotypes between different volunteers before vaccination. Blue boxes represent the overlap unique clonotypes between different volunteers after vaccination.

Table 3. The top 20 high-frequency clonotypes before and after RABV vaccination

	Prevaccination		Postvaccination
Rank	Clonotype sequence	Frequency(%)	Clonotype sequence	Frequency(%)
1	CSVRTVQETQYF	5.78	CSVRTVQETQYF	4.68
2	CSVEDFSSRGFTDTQYF	2.90	CASGYGKELFF	2.49
3	CASGYGKELFF	2.72	CSVEDFSSRGFTDTQYF	1.79
4	CASSVGGGPQETQYF	2.53	CASSVGGGPQETQYF	1.21
5	CASSLGNTGELFF	1.48	CASSLGNTGELFF	0.91
6	CASSEEGMGTYEQYF	1.08	CASSGGQGGTEAFF	0.66
7	CASSSQLDRGETQYF	0.93	CASSEEGMGTYEQYF	0.54
8	CSVERGGGETQYF	0.68	CSVERGGGETQYF	0.48
9	CASSGGQGGTEAFF	0.68	CASSKQGTTEAFF	0.37
10	CASSDASG_HHNSPLHF	0.66	CASSSDRARYGYTF	0.35
11	CASSLEVNTEAFF	0.61	CATSRDHARTEAFF	0.34
12	CSVSGRYEQYF	0.59	CASSRPGQGNTEAFF	0.32
13	CASSSDRARYGYTF	0.58	CASSDASG_HHNSPLHF	0.31
14	CAISESGGVYF	0.56	CASSESHKDTGELFF	0.29
15	CSARVGEGTAYPYEQYF	0.52	CASSSQLDRGETQYF	0.29
16	CASSKQGTTEAFF	0.49	CASSDTKNTGELFF	0.25
17	CASSRPGQGNTEAFF	0.40	CAISESGGVYF	0.24
18	CASSDTKNTGELFF	0.39	CATSREGGETQYF	0.24
19	CATSRDHARTEAFF	0.38	CASSLASGANSPLHF	0.23
20	CATSSGTGVTEAFF	0.36	CAISESGQDQHF	0.23