Figures & data
Table 1. The detail description of the sequence information
Figure 1. Bioinformatics analysis process
Figure 2. Clonal distribution of B cells receptor in pre and post vaccination. (A) Data show the distribution of B-cell clonal frequencies through the measurement of amino acid diversity. Each line represented a volunteer’s diversity changes. (B) Percentage frequency of top 500 BCR nucleotide sequence in pre and post vaccination. The line represents the percentage of top 500 BCR sequence in the total repertoire of each volunteer. (C) Percentage of productive unique BCR nt sequence in volunteers. The line represents the percentage of unique sequence in the total productive B-cell receptor repertoire before and after vaccination. (D) Frequency distribution of BCR nucleotide sequence from volunteers. Data are represented as mean±SEM of distribution of volunteers. The differences between groups were compared using one-way ANOVA. *P < .05. Abbreviation: nt, nucleotide; aa, amino acid
Figure 3. V gene and J gene usage of clonotypes in the volunteers. Data show the percentage frequency of J gene (IGHJ1, IGHJ2, IGHJ3, IGHJ4, IGHJ5 and IGHJ6) and V gene usage by clonotypes in volunteers. Data show mean ±SEM frequency of each individual. Each dot represents each individual volunteer’s information. Data were compared to pre-vaccine using paired t tests. **P < .01 and *P < .05
Figure 4. The usage frequencies of all V-J pairing in the B cell receptor repertoires A-D represent the individual volunteer’s (V1-V4) V-J pairing frequencies. The x axis represents the J genes and the y axis represents the V genes. The area of the circle is proportional to the frequency of each V-J pairing
Figure 5. The amount and frequency of sequences that overlap in the repertoires of pre- and post-vaccination with rabies virus vaccines in 4 volunteers
Table 2. Characteristics of high frequency CDR3 sequences (>0.4%) in the B cell receptor repertoires after immunization with rabies virus vaccines in 4 volunteers