https://orcid.org/0000-0003-1787-1657
Figures & data
Figure 1. Antibody titer responses in mice immunized with the M3-TT, the M6-TT, and the M3/6-TT vaccines. (a) Experiment timeline. (b) Serum samples were collected 14 days after each immunization. Mean titers (± S.E.M.). *P < .01 Significant effects from the second booster compared to the 1st booster, &P < .01 Significant effects entre M3/6-TT vaccine compared M3-TT and M6-TT vaccine. #P < .01 Significant effects entre M3-TT vaccine compared M6-TT vaccine as determined by repeated-measures two-way ANOVA followed by Tukey’s test
Figure 2. The morphine-treated groups had a dose-dependent increase in tail-flick and hot-plate latency. (a) Experiment timeline. Vaccinated mice administered with 1, 10, 20, or 40 mg/kg morphine had an attenuation in morphine-induced tail-flick (b) or hot-plate (c) latency. Mean tail-flick or hot-plate latency (± S.E.M.). *P < .01 significant effects on tail-flick or hot-plate latency in the TT + MOR, the M3-TT-MOR, the M6-TT-MOR, and M3/6-TT-MOR groups compared to saline-treated groups. ** P < .01 Significant effects on tail-flick or hot-plate latency in the TT-MOR groups dosed with 1, 10, or 20 mg/kg morphine compared to the M3-TT-MOR, the M6-TT-MOR, and M3/6-TT-MOR groups. & P < .01 Significant effects on tail-flick or hot-plate latency in the TT-MOR−10mg M3-TT-MOR−10mg, M6-TT-MOR−10mg, and M3/6-TT-MOR−10mg, groups compared to the TT-MOR-1mg, M3-TT-MOR−1mg, M6-TT-MOR−1mg, and M3/6-TT-MOR−1mg groups. # P < .01 Significant effects on tail-flick or hot-plate latency TT-MOR−20mg, M3-TT-MOR−20mg, M6-TT-MOR−20mg, and M3/6-TT-MOR−20mg groups compared to the TT-MOR-10mg, M3-TT-MOR−10mg, M6-TT-MOR−10mg and, M3/6-TT-MOR−10mg groups. ¤ P < .01 Significant effects on tail-flick or hot-plate latency in the TT-MOR−40mg, M3-TT-MOR−40mg, M6-TT-MOR−40mg, and M3/6-TT-MOR−40mg groups compared to the TT-MOR-20mg, M3-TT-MOR−20mg, M6-TT-MOR−20mg, and, M3/6-TT-MOR−20mg groups, as determined by two-way ANOVA followed by Tukey’s test
Table 1. Comparison between the different groups of animals immunized with TT, M3-TT, M6-TT, and M3/6-TT vaccines on antinociception (tail-flick latency) induced by different doses of morphine, using a Tukey test. Groups administered with A) 1 mg/Kg, B) 10 mg/Kg, C) 20 mg/Kg, and D) 40 mg/Kg of morphine are shown in the different panels. *indicates significant p-values (p < .05)
Table 2. Comparison between the different groups of animals immunized with TT, M3-TT, M6-TT, and M3/6-TT vaccines on antinociception (hot-plate latency) induced by different doses of morphine, using a Tukey test. Groups administered with A) 1 mg/Kg, B) 10 mg/Kg, C) 20 mg/Kg, and D) 40 mg/Kg of morphine are shown in the different panels. *indicates significant p-values (p < .05)
Table 3. Comparison between the different groups of animals immunized with TT, M3-TT, M6-TT, and M3/6-TT vaccines on antinociception (tail-flick latency) induced by different doses of morphine, using a Tukey test. Groups administered with A) 1 mg/Kg, B) 2 mg/Kg, C) 5 mg/Kg, and D) 10 mg/Kg of heroin are shown in the different panels. *indicates significant p-values (p < .05)
Table 4. Comparison between the different groups of animals immunized with TT, M3-TT, M6-TT, and M3/6-TT vaccines on antinociception (hot-plate latency) induced by different doses of morphine, using a Tukey test. Groups administered with A) 1 mg/Kg, B) 2 mg/Kg, C) 5 mg/Kg, and D) 10 mg/Kg of heroin are shown in the different panels. *indicates significant p-values (p < .05)
Table 5. Comparison between the different groups of animals immunized with TT, M3-TT, M6-TT, and M3/6-TT vaccines on antinociception (tail-flick or hot-plate latency) induced by different doses of morphine or heroin, using the Pearson correlation coefficient. Panel A shows the Pearson correlation coefficient between morphine-induced tail flick latency and antibody titers. In B) the correlation coefficient between morphine-induced hotplate latency and antibody titers is shown. C) shows the correlation coefficient between heroin-induced tail-flick latency and antibody titers and D) shows the correlation coefficient between heroin-induced hotplate latency and antibody titers. *indicates significant p-values (p < .05)
Figure 3. The heroin-treated groups had a dose-dependent increase in tail-flick and hot-plate latency. (a) Experiment timeline. Vaccinated mice administered with 1, 2, 5, or 10 mg/kg heroin had an attenuation in heroin-induced tail-flick or hot-plate latency. Mean tail-flick (b) or hot-plate (c) latency (± S.E.M.). *P < .01 significant effects on tail-flick or hot-plate latency in the TT + HER, the M3-TT-HER, the M6-TT-HER, and M3/6-TT-HER groups compared to saline-treated groups. ** P < .01 Significant effects on tail-flick or hot-plate latency in the TT-HER groups dosed with 1, 2, 5, or 10 mg/kg heroin compared to the M3-TT-HER, the M6-TT-HER, and M3/6-TT-HER groups. & P < .01 Significant effects on tail-flick or hot-plate latency in the TT-HER-2mg M3-TT-HER−2mg, M6-TT-HER−2mg, and M3/6-TT-HER−2mg compared to the TT-HER-1mg, M3-TT-HER−1mg, M6-TT-HER−1mg, and M3/6-TT-HER−1mg groups. # P < .01 Significant effects on tail-flick or hot-plate latency TT-HER−5mg, M3-TT-HER−5mg, M6-TT-HER−5mg, and M3/6-TT-HER−5mg groups compared to the TT-HER-2mg, M3-TT-HER−2mg, M6-TT-HER−2mg and, M3/6-TT-HER−2mg groups. ¤ P < .01 Significant effects on tail-flick or hot-plate latency in the TT-HER−10mg, M3-TT-HER−10mg, M6-TT-HER−10mg, and M3/6-TT-HER−10mg groups compared to the TT-HER−5mg M3-TT-HER−5mg, M6-TT-HER−5mg, and, M3/6-TT-HER−5mg groups, as determined by two-way ANOVA followed by Tukey’s test
