https://orcid.org/0000-0003-1787-1657
Figures & data
Figure 1. Experiment timeline (a). Antibody titer responses (to the sixth boost) in rats immunized with the TT or COC-TT vaccine (b). Serum samples were collected 14 days after each immunization. Mean titers (± S.E.M.). *p < .01 significant effects of the antibody titers generated by the COC-TT vaccine to the 6th booster compared to the antibody titers generated by the TT vaccine in wistar rats. The COC-TT vaccine reduces cocaine self-administration. Total cocaine seeking during re-acquisition (c). Mean responses on the active and inactive lever during the re-acquisition period (d). *p < .01 significant effects on the active and inactive lever responses in the TT + COC and COC-TT + COC groups compared to saline-treated groups. #p < .01 significant effects on the active and inactive lever responses in the TT + COC group compared to the COC-TT + COC group. As determined by two-way ANOVA followed by Tukey’s tests.
Figure 2. COC-TT vaccine reduces the number of cells immunoreactive to c-fos. Number of cells immunoreactive to c-fos (±S.E.M.) by group (n = 8 animals per group) in the infralimbic cortex (a), AcbC (b), AcbSh (c), and ventral-tegmental area (d). *p < .01 significant effects on the number of cells immunoreactive to c-fos in the TT + COC group compared to the TT + SAL group. #p < .01 significant effects between the TT + COC and COC-TT + COC groups, as determined by two-way ANOVA followed by Tukey’s tests.
Figure 3. Representative photomicrographs of Fos protein expression in the IL, AcbS, AcbC, and VTA of rats from all experimental groups at 20× magnification, where Fos protein expression was visible as dark ovals (highlighted by arrows). Scale bar = 100 μm.
Figure 4. COC-TT vaccine attenuated the re-acquisition of cocaine-induced CPP. Experiment timeline (a). Antibody titer responses (to the sixth boost) in rats immunized with the TT or COC-TT vaccine (b). Mean titers (± S.E.M.). *p < .01 significant effects of the antibody titers generated by the COC-TT vaccine to the 6th booster compared to the antibody titers generated by the TT vaccine in wistar rats. Mean time spent in the cocaine-paired chamber (± S.E.M.) by group (n = 8 animals per group), during acquisition (c) and re-acquisition (d). *p < .01 significant effects on the time in the cocaine-paired side in the TT + COC and COC-TT + COC groups compared to the TT + SAL and COC + SAL groups. #p < .01 significant effects between the TT + COC and COC-TT + COC groups, as determined by two-way ANOVA followed by Tukey’s tests.
