Genetically modified DP915635 maize is agronomically and compositionally comparable to non-genetically modified maize

Figures & data

Table 1. Description of agronomic characteristics evaluated.

Characteristic Measured	Evaluation Timing^a	Description (units of measure)
Early Stand Count	V2-V4	Number of plants emerged per plot (count/m^2)
Days to Flowering	Approximately 50% pollen shed	Number of days from planting until approximately 50% of the plants have tassels shedding pollen (days)
Pollen Viability ^b	During pollen shed	Pollen Shape: pollen grains with collapsed walls at 0, 30, 60, and 120 min (% of pollen with collapsed walls)
		Pollen Color: pollen grains with intense yellow color at 0, 30, 60, and 120 min (% of pollen yellow in color)
Plant Height	R4	Height from soil surface to the collar of flag leaf (cm)
Days to Maturity	R6	Number of days for the majority of the plants to first reach physiological maturity (days)
Lodging	R6	Combined score of stalk lodging (the number of plants in each plot with stalks broken below the primary ear) and root lodging (the number of plants in each plot with stalks leaning approximately 45 degrees or more) values (percentage)
Final Stand Count	R6	Number of plants remaining per plot (count/m^2)
Dropped Ears	R6	Number of ears lying on the ground within each plot (count)
Yield	R6	Harvest weight per area adjusted to 15.5% moisture content (bushels per acre)
Harvest Grain Moisture	R6	Grain moisture content (percentage)
100-Kernel Weight	R6	Weight of 100 kernels (grams)

^aAbendroth et al. ³⁷ describes maize growth stages.

^bPollen viability has been correlated to pollen shape and color ²⁸.

Table 2. Across-site analysis results for agronomic characteristics that had statistically significant differences between DP915635 maize and non-GM near-isoline control maize (control).

Agronomic Characteristic	Reported Statistics^a	Control Maize	DP915635 Maize	Reference Range^c
Early Stand (count/m^2)	Mean	5.8	5.9	4.1–6.5
	Range	4.6–6.4	4.7–6.4
	Confidence Interval	5.5–6.2	5.6–6.3
	Adjusted P-Value	–	0.0812
	P-Value	–	<0.00541*
Days to Flowering (days)	Mean	63.9	63.5	54–74
	Range	55–75	56–76
	Confidence Interval	59.5–68.3	59.1–67.9
	Adjusted P-Value^b	–	0.209
	P-Value^b	–	0.0279*

^aThe across-site statistical analysis for each agronomic characteristic included results from all ten field sites with four replicate blocks at each site.

^bP-values < 0.05 are denoted by *. Adjusted P-values were used to control for false positive outcomes across all agronomic characteristics analyzed using linear mixed models.

^cReference ranges were obtained from four non-GM commercial maize lines grown at each site in the study.

Table 3. Across-site analysis results for composition analytes that had statistically significant differences between DP915635 maize and non-GM near-isoline control maize (control).

Composition Analyte^a	DP915635 Mean (Range)	Control Mean (Range)	P-value (Adjusted P-value)^b	Reference Maize Range^c	Tolerance Interval^d	Literature Range^e
Palmitoleic acid (C16:1)	0.122 (0.0999–0.140)	0.119 (0.0940–0.145)	0.0178* (0.289)	0.0911–0.184	0–0.435	NQ − 0.67
Stearic acid (C18:0)	2.02 (1.75–2.64)	1.98 (1.74–2.44)	0.0297* (0.301)	1.73–2.92	1.32–3.69	NQ − 4.9
Oleic acid (C18:1)	2.38 (22.0–25.0)	24.1 (22.2–25.6)	0.00864* (0.204)	21.0–32.2	16.9–38.4	16.38–42.81
Linoleic acid (C18:2)	5.92 (56.9–61.4)	58.8 (56.6–61.4)	0.00822* (0.204)	49.2–61.5	31.9–65.3	13.1–67.68
Lignoceric acid (C24:0)	0.294 (0.261–0.367)	0.301 (0.270–0.376)	0.0203* (0.289)	0.250–0.451	0–0.622	NQ − 0.91
Methionine	0.200 (0.147–0.260)	0.214 (0.165–0.292)	0.0287* (0.301)	0.151–0.255	0.106–0.315	0.10–0.47
α-tocopherol	3.97 (<0.500^f − 9.97)	3.67 (<0.500^f − 9.83)	0.00147* (0.104)	<0.500^f − 25.2	0–22.9	NQ − 68.67

^aThe across-site statistical analysis for each analyte included results from all eight field sites with four replicate blocks at each site.

^bP-values < 0.05 are denoted by *. Adjusted P-values were used to control for false positive outcomes across all analytes analyzed using linear mixed models.

^cReference ranges were obtained from four non-GM commercial maize lines grown at each site in the study.

^dTolerance intervals were derived from Corteva Agriscience’s™ proprietary accumulated data from commercial non-GM maize lines.

^eLiterature ranges were obtained from published literature.^19,23–27 NQ; not detectable: one or more assay values in the published literature references were below the lower limit of quantification (LLOQ) and were not quantified.

^f< LLOQ, one or more sample values were below the assay LLOQ.

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