Figures & data
Figure 1. Metabolic pathway and genetic modification strategy for P3HP production by recombinant K. pneumoniae strain from glycerol. DhaB: glycerol dehydratase; AldH: aldehyde dehydrogenase; PrpE: propionyl-CoA synthetase; PhaC: polyhydroxyalkanoate synthetase; DhaT and YqhD: 1,3-propanediol oxidoreductases.
Figure 2. SDS-PAGE analysis of cell-free soluble extract of Q1533, Q1546 and Q1643. The arrows indicate PrpE～70KDa, DhaB1 and GdrA～62KDa, AldH～55KDa, PhaC～40KDa, DhaB2～21KDa, DhaB3 and GdrB～16KDa. M-protein maker, 1-Q1533, 2-Q1546 and 3-Q1643.
Figure 3. Production of 3HP by different recombinant strains. The strains were grown in minimal medium under shake flask condition in triplicate, and standard deviation was shown.
Figure 4. The 600 MHz 1H(A) and 13C(B) NMR spectra of P3HP synthesized by Q1643 in CDCl3 solution. The chemical shift assignment for each proton and carbon resonance was showed.
Table 1. Production of P3HP under different aeration
Table 2. Bacterial strains, plasmids, and primers used in this study