A thermostable archaeal S-adenosylmethionine synthetase: a promising tool to improve the synthesis of adenosylmethionine analogs of biotechnological interest

Figures & data

Table 1. Effect of several water-miscible organic solvents on the stability of PfMAT at 50°C and 60°C. The enzyme was incubated in stoppered glass tubes in 10 mM Tris-HCl, pH 8.0, with the compounds to be tested. At the time indicated, aliquots were removed from each incubation mixture and assayed for the residual PfMAT activity in standard conditions at 80°C

	Residual activity
Compound	50°C				60°C
	15 min	30 min	60 min		15 min	30 min	60 min
	%					%
None	100	100	100		100	100	100
50% methanol	100	100	100		59	28	16
50% ethanol	118	92	86		57	18	6
50% n-propanol	36	22	13		33	12	8
50% isopropanol	66	50	28		26	13	8
50% acetonitrile	90	89	80		73	64	42
50% DMSO	100	100	100		100	100	100

Figure 1. Effect of increasing concentrations of methanol, ethanol, acetonitrile, and DMSO on the acivity of PfMAT at 50°C. The enzyme was assayed in standard conditions for 15 min in the presence of the solvent to be analyzed at different percentage from 5% to 30% (vol/vol). (♦) Methanol; (▪) ethanol; (▴) acetonitrile; (•) DMSO. Each error bar indicates the standard deviation (± SD). Values were the mean of triplicate analyses.