New biotechnological applications for Ashbya gossypii: Challenges and perspectives

Figures & data

Figure 1. Main products and metabolic reactions involved in the new biotechnological applications for which A. gossypii has been explored. Dashed arrows indicate several reactions. DHAP, dihydroxy-acetone-phosphate; PRPP, phosphoribosyl pyrophosphate; IMP, inosine-5′-phosphate; GMP, guanosine-5′-phosphate; GTP, guanosine-5′-triphosphate.

Table 1. Production levels of some biotechnological relevant compounds produced by A. gossypii. The production levels of these bioproducts by other microorganisms are also presented for comparison.

Species	Product	Genetic Background	Culture Conditions Optimization	Production levels	Reference(s)
Recombinant proteins
A. gossypii	Endoglucanase I	Engineered	No	Up to 400 μmol/min.L	^14,16
Saccharomyces cerevisiae		Engineered	No	Up to 1000 μmol/min.L
A. gossypii	β-galactosidase	Engineered	No	Up to 1127 U/mL	^17
Aspergillus niger		Wild Type	No	Up to 3000 U/mL
SCOs
A. gossypii	Single cell oil	Engineered	No	Up to 70% of CDW	^10
Yarrowia lipolytica		Engineered	Yes	Up to 88% of CDW
A. gossypii	Linoleic acid	Engineered	No	16% of total fatty acids	^18
Y. lipolytica		Wild type	Yes	31% of total fatty acids	^19
Flavour compounds
A. gossypii	2-phenylethanol	Wild type	No	Up to 0.2 g/L	^11
S. cerevisiae and Kluyveromyces marxianus		Wild type	Yes	0.25 – 20 g/L	^20
A. gossypii	Isoamyl alcohol	Engineered	No	0.5 – 0.6 g/L	^11
S. cerevisiae		Mutants	Yes	0.4 – 0.5 g/L	^21
A. gossypii	Inosine	Engineered	No	Up to 2.2 g/L	^12,13
Bacillus subtilis		Engineered	No	6 – 7 g/L
A. gossypii	Guanosine	Engineered	No	Up to 0.14 g/L	^12
Bacillus amyloliquefaciens		Engineered mutant	No	Up to 13.5 g/L	^22

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