Figures & data
Figure 1. The construction of human ALIM infected by Streptococcus. A. The relative LDH vitality of supernates in ALIM at 4 h, 24 h and 48 h. B. The relative expression of IL-6, IL-8 and TNF-α of supernates in ALIM. LDH, lactate dehydrogenase; IL, interleukin; TNF, tumor necrosis factor; ALIM, acute lung infection model; cfu, colony-forming unit.*, P < 0.05, **, P < 0.01.
Figure 2. Alterations of calpains activity in the ALIM supernates with the calpain inhibitor (calpeptin). A. Calpain activity in the ALIM supernate maintained on a high level. B. The effect of different doses of calpeptin on calpains activity. C. Expressions of IL-6, IL-8 and TNF-α in ALIM were significantly reduced by calpeptin. D. The expression of PCNA protein was lower when the calpeptin was added. RFU, relative fluorescence units; NC, negative control; PCNA, proliferating cell nuclear antigen; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL, interleukin; TNF, tumor necrosis factor; ALIM, acute lung infection model. *, P < 0.05, **, P < 0.01.
Figure 3. The effect of calpains on the lung cells MRC-5. A. The transfection efficiency of siRNA-calpains 1 and 2 to lung fibroblast MRC-5 cells. B. Expressions of IL-6, IL-8 and TNF-α in transferred lung cells with calpains silence. C. The expressions of PCNA protein in transferred lung cells with calpains silence. NC, negative control; si, small interfering; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL, interleukin; TNF, tumor necrosis factor; PCNA, proliferating cell nuclear antigen. *, P < 0.05.
Figure 4. The effect of calpains on membrane permeability and cell apoptosis in the lobar pneumonia. A. LDH release was used to detect cell membrane permeability in MRC-5 cells. B. The flow cytometry identified cell apoptosis in MRC-5 cells with calpains silence. NC, negative control; si, small interfering; LDH, lactate dehydrogenase; PI, Propidium iodide.*, P < 0.05.
Table 1. Characteristics and demographics in 5 patients undergoing lung resection.