Figures & data
Figure 1. Effects of transfection on SCs. SCs were transfected with S100A4 vector or siRNA against S100A4. (A) The morphology of the transfected SCs was observed and photographed under a light-microscope. (B) The mRNA level expression of S100A4 in transfected SCs was determined by qPCR. (C) The protein level of S100A4 was detected by Western blotting. SCs, Schwann cells; siRNA, small interfering RNA; qPCR, quantitative PCR; **, P < 0.01.
Figure 2. Effects S100A4 on SCs migration. SCs were transfected with S100A4 vector or siRNA against S100A4. (A) SCs migration was measured by Tranwell assay, and the migrated SCs were stained with crystal violet and photographed under a light-microscope. (B) Quantification of migrated SCs. (C) Protein expressions of VEGF and MMP-9 in transfected SCs were measured by Western blot. SCs, Schwann cells; siRNA, small interfering RNA; VEGF, vascular endothelial growth factor precursor; MMP-9, matrix metallopeptidase 9; **, P < 0.01; ***, P < 0.001.
Figure 3. Effects of dysregulated S100A4 SCs on the expression of GAP43 in SGNs. SGNs were co-cultured either with S100A4 overexpressed or suppressed SCs. (A) GAP43 in SGNs was probed by IF and observe under an automated upright microscope system. (B) The mRNA level expression of GAP43 in SGNs was detected by qPCR. (C) The protein expression of GAP43 was detected by Western blot. SCs, Schwann cells; SGNs, spiral ganglion neurons; GAP43, growth associated protein 43; IF, immunofluorescence; qPCR, quantitative PCR; *, P < 0.05; **, P < 0.01.
