Application of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus

Figures & data

Figure 1. (a) Protein concentration in the control and experimental groups (by month).(b) Indirect ELISA of the M2e-specific IgY antibody.

Figure 2. Schematic illustration of the antibody-enzyme conjugation method.

Figure 3. (a) Fluorescence spectrum of the M2e-specific IgY antibody. (b) Fluorescence spectrum of the ALP enzyme. (c) Fluorescence spectrum of the M2e-specific IgY-ALP bioconjugate. (d) A comparison of the fluorescence spectra following conjugation reaction.

Figure 4. FTIR spectrum of the M2e-specific IgY antibody.

Figure 5. FTIR spectrum of the ALP enzyme.

Figure 6. FTIR spectrum of the M2e-specific IgY-ALP bioconjugate.

Figure 7. Sandwich ELISA of M2e peptide antigens in nasopharyngeal specimens. (I: H3N2; II: H1N1), Error bars indicate the standart deviation. Mean value of negative control is 0.2184, and cut-off value is 0.257.

Table 1. Primer-probe sequences.

Primers	Sequences
Influenza A Forward	GAC CRA TCC TGT CAC CRC TGA C
Influenza A Reverse	AGG GCA TTY TGG ACA AAK CGT CTA
Influenza A Probe1	TGC AGT CCT CGC TCA CTG GGC ACG
Influenza A/pdmH1 Forward	GTG CTA TAA ACA CCA GCC TYC CA
Influenza A/pdmH1 Reverse	CGG GAT ATT CCT TAA TCC TGT RGC
A/H1pdm Probe2	CA GAA TAT ACA ‘T’ CC RGT CAC AAT TGG ARA A
Influenza A/H3 Forward	AGCAAAGCCTACAGCAA
Influenza A/H3 Reverse	GACCTAAGGGAGGCATAA
A/H3 Probe1	CCGGCACATCATAAGGGTAACA
RnaseP Forward	AGA TTT GGA CCT GCG AGC G
RnaseP Reverse	GAG CGG CTG TCT CCA CAA GT
RnaseP Probe	TTC TGA CCT GAA GGC TCT GCG CG

Figure 8. Schematic illustration of the sandwich ELISA method.