A genome-wide survey of interaction between rice and Magnaporthe oryzae via microarray analysis

Figures & data

Table 1. Genes chosen for confirmation by qRT-PCR

Locus ID	Description	Fold change
		24hpi	48hpi	72hpi
LOC_Os11g01480.1	MYB family transcription factor	2.3	3.8	10.5
LOC_Os10g28080.1	Similar to Chitinase 1 precursor	2.2	11.7	21.6
LOC_Os04g03830.1	Serine/threonine protein kinase-related domain containing protein.	2.2	3.0	11.0

Figure 1. The expression level of the genes chosen for confirmation by QRT-PCR at three time points

Figure 2. Trend analysis and annotation of the 115 significantly differentially expressed genes. (a) Using stem software to cluster the change trend of the 115 genes during the three time points. Marked color chart is a significant trend, such as the thirteenth and fifteenth diagram. (b) Three categories of the significant trend genes according to different functions by AgriGO database. C, F and P corresponding to cellular component, molecular function, and biological process, respectively. (c) KEGG analysis of the significant trend genes

Table 2. The expression of rice defense-related genes

Locus ID	Description	Fold change
		24hpi	48hpi	72hpi
Pathogen recognition
LOC_Os04g30260.1	OsWAK47 – OsWAK receptor-like protein kinase	3.2	2.7	18.5
LOC_Os09g29510.1	OsWAK80 – OsWAK receptor-like protein kinase	2.0	3.4	11.4
LOC_Os04g29580.1	OsWAK37 – OsWAK short gene	2.7	2.2	16.1
LOC_Os04g30250.3	wall-associated receptor kinase-like 5 precursor	2.9	2.1	6.3
LOC_Os11g11960.1	Similar to NBS-LRR disease resistance protein homologue	2.2	2.6	10.6
LOC_Os11g12320.1	Similar to NBS-LRR protein	2.0	2.2	11.3
LOC_Os11g12040.1	Similar to NBS-LRR protein	2.0	2.1	5.6
LOC_Os06g10790.1	lectin-like receptor kinase	3.2	2.4	8.0
Signal Transduction
LOC_Os01g50420.1	Serine/threonine protein kinase domain containing protein	0.2	76.4	829.3
LOC_Os04g03830.1	Serine/threonine protein kinase-related domain containing protein	2.2	3.0	11.1
Transcriptional regulation
LOC_Os11g01480.1	Myb-like DNA-binding domain, SHAQKYF class family protein	2.3	3.8	10.5
LOC_Os08g36920.1	Similar to AP2 domain containing protein RAP2.6	0.1	16.6	136.7
Defense
LOC_Os10g28080.1	Similar to Chitinase 1 precursor	2.2	11.7	21.6
Biochemical metabolism
LOC_Os12g24320.1	ATPase 3, AAA-type, core domain containing protein	3.2	15.6	246.6
LOC_Os01g38110.1	Cytochrome P450 family protein	2.3	21.7	53.5
LOC_Os12g39310.1	Cytochrome P450 family protein	0.2	3.5	31.8
LOC_Os08g39730.1	Oryza sativa Cyt-P450 monooxygenase (PM-II) mRNA, complete cds	0.4	16.7	8.7
LOC_Os04g10160.1	Similar to Cytochrome P450 CYP99A1	5.0	28.9	154.6

Table 3. The expression of rice novel defense-related genes

Locus ID	Description			Fold change
				24hpi	48hpi	72hpi
LOC_Os01g02130.1		Conserved hypothetical protein		2.2	2.8	13.5
LOC_Os01g48620.1			Conserved hypothetical protein	2.7	2.3	48.1
LOC_Os01g60930.1			Conserved hypothetical protein	2.89	2.69	8.89
LOC_Os06g38660.1			Conserved hypothetical protein	2.2	18.6	221.0
LOC_Os09g04310.1			Conserved hypothetical protein	2.7	3.3	15.0
LOC_Os12g31540.1			Conserved hypothetical protein	3.5	2.8	18.5
LOC_Os04g03164.1			Hypothetical conserved gene	2.2	6.5	36.9
LOC_Os04g22120.1			Hypothetical conserved gene	2.9	6.4	13.2
LOC_Os07g31190.1			Hypothetical conserved gene	2.7	2.7	12.0
LOC_Os11g38790.2			Hypothetical conserved gene	3.0	4.7	58.4
LOC_Os07g03040.2			Protein of unknown function DUF1719, Oryza sativa family protein	2.77	7.07	53.0
LOC_Os12g33300.1			Protein of unknown function DUF6, transmembrane domain containing protein	2.2	2.7	26.4
LOC_Os01g09220.1			Conserved hypothetical protein	0.2	2.6	15.1
LOC_Os01g50350.2			Conserved hypothetical protein	0.2	6.1	27.8
LOC_Os01g62190.1			Conserved hypothetical protein	0.3	6.1	23.8
LOC_Os05g47960.1			Conserved hypothetical protein	0.3	3.5	9.2
LOC_Os09g17146.1			Conserved hypothetical protein	0.5	3.5	149.2
LOC_Os12g05690.1			Conserved hypothetical protein	0.4	9.3	108.2
LOC_Os06g02830.1			Hypothetical conserved gene	0.3	0.4	0.2
LOC_Os03g08980.1			Conserved hypothetical protein	3.5	2.9	0.4
LOC_Os03g10250.1			Conserved hypothetical protein	0.4	6.5	0.1