Analysis of Collagen type X alpha 1 (COL10A1) expression and prognostic significance in gastric cancer based on bioinformatics

Figures & data

Table 1. Characteristics of patients with gastric cancer in GSE84437 dataset

Characteristics	Variable	Patients (433)	Percentages (%)
Age	<65 years ≥65 years	267 166	61.66 38.34
Gender	Male Female	296 137	68.36 31.64
T classification	T1 T2 T3 T4	11 38 92 292	2.54 8.78 21.24 37.44
N classification	N0 N1 N2 N3	80 188 132 33	18.48 43.42 30.48 7.62
Vital status	Alive Death	224 209	51.73 48.27

Figure 1. Expression level of COL10A1 in GC and its relationship with pathological parameters. The mRNA expression of COL10A1 in GC (a), diffuse gastric adenocarcinoma (b) and gastric intestinal-type adenocarcinoma (c, d) compared to normal individuals derived from the Oncomine database. Comparison of COL10A1 expression between GC tissues and adjacent nontumor tissue base on TCGA data (e). The expression of COL10A1 is grouped by tumor differentiation (f), pathological stage (g) and T stage (h)

Table 2. Relationship between COL10A1 expression and clinicopathological parameters in gastric cancer

Clinicopathological parameters	COL10A1 expression
	Hight (n = 159)	Low (n = 158)	Total	P-value
Age <65 ≥65	66(49.6) 93(50.5)	67(50.4) 91(49.5)
Gender Male Female	102(51.5) 57(47.9)	96(48.5) 62(52.1)	198 119	0.611
Grade G1&G2 G3	50(43.5) 109(54.0)	65(56.5) 93(46.0)	115 202	0.093
Pathological stage I&II III&IV	75(51.7) 84(48.8)	70(48.3) 88(51.2)	145 172	0.689
T classifification T1–T2 T3–T4	31(38.8) 128(54.0)	49(61.2) 109(46.0)	80 237	0.025
Lymph node metastasis Negative Positive	55(54.5) 104(48.1)	46(45.5) 112(51.9)	101 216	0.025
Distant metastasis No Yes	148(50.0) 11(52.4)	148(50.0) 10(47.6)	296 21	1

Table 3. COL10A1 expression correlated with clinicopathological parameters

Clinicopathological parameters	Total (N)	Odds ratio in COL10A1 expression	P-value
Age ≥65 vs. <65	371	1.035(0.687–1.559)	0.870
Gender Male vs. female	375	1.088(0.713–1.662)	0.694
Tumor differentiation G3 vs. G2&G1	366	1.543(1.015–2.356)	0.043
Pathological stage Stage II vs. Stage I Stage III vs. Stage I Stage IV vs. Stage I	164 203 91	3.106 (1.577–6.307) 2.062(1.079–4.062) 2.118(0.902–5.061)	0.001 0.031 0.086
T classification T2 vs. T1 T3 vs. T1 T4 vs. T1	99 187 119	14.727(2.827–271.308) 21.273(4.243–387.099) 22(4.286–403.417)	0.011 0.003 0.003
Lymph node metastasis Positive vs. negative	357	0.870(0.555–1.363)	0.543
Distant metastasis Yes vs. no	355	0.923(0.404–2.094)	0.847

Figure 2. Survival analysis, univariate and multivariate analysis. Kaplan-Meier curve of the relationship between COL10A1 mRNA expression and the prognosis of GC patients based on Kaplan-Meier plots database (a) and GSE84437 dataset (d). Univariate and multivariate analysis of COL10A1 expression and its correlation in patients with GC base on TCGA data (b, c) and GSE84437 dataset (e, f)

Table 4. Correlation of COL10A1 mRNA expression and clinical prognosis in gastric cancer

	Overall survival	Post progression survival
	N HR p-Value	N HR p-Value
Sex Female Male	236 1.94(1.32–2.77) 0.00047 544 1.35(1.06–1.71) 0.013	149 1.48(0.95–2.29) 0.081 348 1.27(0.98–1.64) 0.074
Stage 1 2 3 4	69 0.11(0.02–0.49) 0.00046 140 0.65(0.34–1.23) 0.18 305 1.90(1.41–2.57) 1.7e-05 148 1.82(1.23–2.96) 0.0023	31 0.08(0.01–0.72) 0.0051 105 0.71(0.37–1.38) 0.31 142 2.6(1.63–4.14) 3.2e-05 104 1.45(0.89–2.38) 0.13
Stage T 1 2 3 4	14 - - 241 1.36(0.87–2.12) 0.18 204 1.76(1.25–2.49) 0.0011 38 0.56(0.24–1.35) 0.19	3 - - 196 1.25(0.79–1.99) 0.33 150 1.66(1.12–2.44) 0.0099 29 - -
Stage N 0 1 2 3 1 + 2 + 3	76 0.49(0.21–1.12) 0.085 225 1.97(1.30–3.00) 0.0012 121 2.78(1.60–4.85) 0.00016 76 1.79(1.03–3.09) 0.035 422 1.82(1.40–2.37) 6.6e-06	41 0.35(0.11–1.09) 0.059 169 1.61(1.02–2.53) 0.039 105 2.17(1.33–3.56) 0.0016 63 1.6(0.85–2.99) 0.14 337 1.6(1.2–2.13) 0.0011
Stage M 0 1	444 1.74(1.30–2.33) 0.00015 56 1.96(1.05–3.67) 0.33	342 1.51(1.12–2.04) 0.0068 36 2.35(1.04–5.33) 0.035
Lauren classification Intestinal Diffuse Mixed	320 2.20(1.59–3.04) 9.4e-07 241 1.66(1.16–2.36) 0.0049 32 – -	192 1.66(1.09–2.51) 0.0161 176 1.67(1.07–2.58) 0.021 16 – -
Differentiation Poorly differentiated Moderately differentiated Well differentiated	165 2.08(1.27–3.40) 0.0029 67 2.34(1.21–4.53) 0.0098 32 – -	49 1.36(0.72–2.58) 0.3428 24 – - 0 – -
HER2 status HER2 negative HER2 positive	532 1.22(0.96–1.56) 0.11 343 1.74(1.31–2.30) 8.6e-05	334 1.19(0.89–1.59) 0.24 164 1.53(1.04–2.26) 0.03

Figure 3. GSEA analysis and experimental verification. (a) A combined enrichment plot has been obtained from the analysis of the enrichment of the gene series, including the enrichment fraction and gene series (top 10 terms). (b) The expression level of COL10A1 was higher than that of GES-1 in three kinds of GC cells (AGS, SGC-7901 and MGC-803) (*P < 0.05, **P < 0.01). (c) qRT-PCR analysis of COL10A1 mRNA expression in 30 pairs of GC tissues and adjacent nontumor tissues

Availability of data and materials

Data were analyzed from GEO (http://www.ncbi.nlm.nih.gov/geo), TCGA (https://portal.gdc.cancer.gov), Oncomine database (https://www.oncomine.org/resource/login.html) and Kaplan–Meier plots (https://kmplot.com/analysis/). We declare that the data and materials in this study will be provided free of charge to scientists for noncommercial purposes.