https://orcid.org/0000-0001-8917-7176
Figures & data
Figure 1. Expression of miR-487a-3p is downregulated during oral squamous cell carcinoma
(a) Expression of miR-487a-3p in OSCC tissue. (b) Expression of miR-487a-3p in OSCC cell lines TCA8113 and CAL-27. *P < 0.05 and **P < 0.01.
Figure 2. Overexpression of miR-487a-3p impedes cell growth and invasion in OSCC in vitro.
(a) Assessment of the miR-487a-3p mimics transfection in OSCC cell lines. (b,c) CCK-8 analysis with miR-487a-3p mimics or NC in OSCC cell lines. (d)Colony formation analysis for cell growth with miR-487a-3p mimics or NC in OSCC cell lines. (e) Transwell invasion analysis for cell invasion with miR-487a-3p mimics or NC in OSCC cell lines. *P < 0.05 and **P < 0.01.
Figure 3. Targeting of PPM1A by miR-487a-3p in OSCC cells
(a) 3’UTR region of PPM1A containing the binding site for miR-487a-3p in WT and MUT plasmids. (b) Luciferase activity of PPM1A WT or MUT 3’-UTR with miR-487a-3p mimics or NC in OSCC cell lines. (c) The expression of PPM1A mRNA is up-regulated in OSCC cell lines (TCA8113 and CAL-27). (d) The expression of PPM1A mRNA affected with miR-487a-3p mimics or NC in OSCC cell lines. (e) The expression of PPM1A protein affected with miR-487a-3p mimics or NC in OSCC cell lines. *P < 0.05 and **P < 0.01.
Figure 4. Knockdown of PPM1A suppresses OSCC growth, and invasion
(a) Expression of PPM1A protein affected by si-PPM1A siRNAs in OSCC cells. (b,c) CCK-8 analysis with si-PPM1A or NC in OSCC cell lines. (d) Colony formation analysis for cell growth with si-PPM1A or NC in OSCC cell lines. (e) Transwell invasion analysis for cell invasion with si-PPM1A or NC in OSCC cell lines. *P < 0.05 and **P < 0.01.
Figure 5. Mimicking miR-487a-3p rescues the oncogenic effects of PPM1A in OSCC
(a) Expression of miR-487a-3p in OSCC cells under transfection of miR-487a-3p and PPM1A-OE plasmid. (b) Expression of PPM1A protein in OSCC cells under transfection of miR-487a-3p and PPM1A-OE plasmid. (c,d) Cell proliferation in OSCC tissue under transfection of miR-487a-3p and PPM1A-OE plasmid. (e) Colony formation analysis for cell growth under transfection of miR-487a-3p and PPM1A-OE plasmid. (f) Transwell invasion analysis for OSCC cell invasion under transfection of miR-487a-3p and PPM1A-OE plasmid. *P < 0.05 and **P < 0.01.
