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Research Paper

Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism

, , , & ORCID Icon
Pages 640-647 | Received 04 Jan 2021, Accepted 02 Feb 2021, Published online: 15 Feb 2021

Figures & data

Figure 1. Inhibitory effect of PTF from young loquat fruits on PPO

Figure 1. Inhibitory effect of PTF from young loquat fruits on PPO

Figure 2. Consecutive spectra obtained during the oxidation of L-DOPA by PPO. (a), Consecutive spectra in the absence of PTF. (b). Consecutive spectra in the presence of PTF. Lines 1–10 represent 0–9 min after the addition of the enzyme. PTF: 20 μg/mL

Figure 2. Consecutive spectra obtained during the oxidation of L-DOPA by PPO. (a), Consecutive spectra in the absence of PTF. (b). Consecutive spectra in the presence of PTF. Lines 1–10 represent 0–9 min after the addition of the enzyme. PTF: 20 μg/mL

Figure 3. Inhibition mechanism of PTF from young loquat fruits on PPO. The concentrations of PTF for lines 1–5 were 0, 5, 10, 15, and 20 μg/mL

Figure 3. Inhibition mechanism of PTF from young loquat fruits on PPO. The concentrations of PTF for lines 1–5 were 0, 5, 10, 15, and 20 μg/mL

Figure 4. Inhibition type and inhibition constants of TF from young loquat fruits on PPO. The concentrations of TF for lines 1–5 were 0, 5, 10, 15, and 20 μg/mL

Figure 4. Inhibition type and inhibition constants of TF from young loquat fruits on PPO. The concentrations of TF for lines 1–5 were 0, 5, 10, 15, and 20 μg/mL

Figure 5. Effect of PTF from young loquat fruits on the emission spectrum of PPO. (a), Intrinsic fluorescence changes. (b), Florescence intensity changes. (c), Stern–Volmer curves of PTF. (d), Plot of log[(F0 − F)/F] against log[I] for PTF

Figure 5. Effect of PTF from young loquat fruits on the emission spectrum of PPO. (a), Intrinsic fluorescence changes. (b), Florescence intensity changes. (c), Stern–Volmer curves of PTF. (d), Plot of log[(F0 − F)/F] against log[I] for PTF

Figure 6. (a), Three-dimensional fluorescence spectra of PPO in the absence of PTF; (b), Three-dimensional fluorescence spectra of PPO in the presence of PTF (20 μg/mL)

Figure 6. (a), Three-dimensional fluorescence spectra of PPO in the absence of PTF; (b), Three-dimensional fluorescence spectra of PPO in the presence of PTF (20 μg/mL)
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Supplemental Material

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