2,372
Views
12
CrossRef citations to date
0
Altmetric
Research Paper

Yiqi Huoxue Recipe inhibits cardiomyocyte apoptosis caused by heart failure through Keap1/Nrf2/HIF-1α signaling pathway

, , , , , , , , , & show all
Pages 969-978 | Received 01 Feb 2021, Accepted 04 Mar 2021, Published online: 19 Mar 2021

Figures & data

Figure 1. YHR inhibited the area of cardiac infarction. Heart cross section (left) and infarct area (right). Each group consists of three continuous cross sections of rat heart. The ratio of the infarct area to a single section X 100% was used as the infarct rate. The infarction rate is expressed as the mean ± SD of each group. **P  <0.01, ***P < 0.001 vs. Model group

Figure 1. YHR inhibited the area of cardiac infarction. Heart cross section (left) and infarct area (right). Each group consists of three continuous cross sections of rat heart. The ratio of the infarct area to a single section X 100% was used as the infarct rate. The infarction rate is expressed as the mean ± SD of each group. **P  <0.01, ***P < 0.001 vs. Model group

Figure 2. YHR suppressed cell apoptosis of cardiac tissue in HF model

A. Tunel assay was used to detect the apoptosis rate of the infarct marginal zone of the model group (Magnification, 200×). B. The expression of apoptosis-related proteins, including Bcl-2, Bax and caspase-3 in the cardiac tissue was measured by western blot. **P < 0.01 vs. Model group.
Figure 2. YHR suppressed cell apoptosis of cardiac tissue in HF model

Figure 3. YHR rescued the loss of cell viability induced by OGD/R

A. H9C2 cells were incubated with different concentrations of YHR (300, 500, 700 μg/ml). MTT assay was used to detect cell viability. B. The cell apoptosis rate was determined by Hoechst 33342 assay (200×). **P < 0.01 vs. Model group.
Figure 3. YHR rescued the loss of cell viability induced by OGD/R

Figure 4. Detect the changes of YHR on the level of mitochondrial membrane potential (ΔΨm) of H9C2 cells induced by OGD/R

Figure 4. Detect the changes of YHR on the level of mitochondrial membrane potential (ΔΨm) of H9C2 cells induced by OGD/R

Figure 5. YHR attenuates the production of ROS in cardiomyocytes induced by OGD/R

The DCFH-DA assay was used to detect the ROS levels of cells in different groups. During the OGD/R process, YHR down-regulated the production of ROS (100×) compared with the model. ***P < 0.001 vs. model group.
Figure 5. YHR attenuates the production of ROS in cardiomyocytes induced by OGD/R

Figure 6. YHR suppressed the activation of Keap1/Nrf2/HIF-1α pathway

Figure 6. YHR suppressed the activation of Keap1/Nrf2/HIF-1α pathway