Figures & data
Figure 1. The expression of miR-9 was downregulated in AML cell lines. Relative expression of miR-9 was measured in AML cell lines (THP-1, HL-60, TF-1 and KG-1) and human normal bone marrow cells (HS-5) using qRT-PCR. *P < 0.05, **P < 0.01, ***P < 0.0001 vs. HS-5 cell
Figure 2. miR-9 restrain the sharp increase of AML cells and boost their apoptosis. After transfection of miR-9 mimic, NC mimic, miR-9 inhibitor and NC inhibitor, QRT-PCR was used to find out the expression of miR-9; (b) after transfection of miR-9 mimic, NC mimic, miR-9 inhibitor and NC inhibitor, CCK-8 was used to detect the cell viability of HL-60. (c) After transfection of miR-9 mimic, the abrupt increase of HL-60 cells was detected by cell colony assay, NC mimic, miR-9 inhibitor and NC inhibitor; (d) After transfection of miR-9 mimic, NC mimic, miR-9 inhibitor and NC inhibitor, flow cytometry was used to detect the apoptosis rate of HL-60. Compared with NC inhibitor, **P < 0.01; compared with NC mimic, ##P < 0.01
Figure 3. Effect of miR-9 on Hippo/YAP signal pathway in HL-60 cells. After transfection of miR-9 mimic, NC mimic, miR-9 inhibitor, and NC inhibitor, the expression levels of the corresponding proteins MAT1, LATS1, LATS2, and YAP in the Hippo/YAP signaling pathway in HL-60 cells was detected by using the Western blot
