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Research Paper

AGEs/RAGE blockade downregulates Endothenin-1 (ET-1), mitigating Human Umbilical Vein Endothelial Cells (HUVEC) injury in deep vein thrombosis (DVT)

, , , , , & show all
Pages 1360-1368 | Received 29 Jan 2021, Accepted 12 Apr 2021, Published online: 25 Apr 2021

Figures & data

Figure 1. Concentration of AGEs (CML and imidazolone) in M-HSA (ELISA)

Figure 1. Concentration of AGEs (CML and imidazolone) in M-HSA (ELISA)

Figure 2. A HUVEC viability in the blank group, HSA, M-HSA and M-HSA+FPS-ZM1 (CCK-8) **P < 0.01; B-C HUVEC viability in the blank group, HSA, M-HSA and M-HSA+FPS-ZM1 (flow cytometry) ##P < 0.01, ***P < 0.001

Figure 2. A HUVEC viability in the blank group, HSA, M-HSA and M-HSA+FPS-ZM1 (CCK-8) **P < 0.01; B-C HUVEC viability in the blank group, HSA, M-HSA and M-HSA+FPS-ZM1 (flow cytometry) ##P < 0.01, ***P < 0.001

Figure 3. A-C relative expressions of pro-inflammatory cytokines in the blank group, HSA, M-HSA and M-HSA+FPS-ZM1 (ELISA) ***P < 0.001, ###P < 0.001; D-E relative expressions of ROS, MDA and SOD in the blank group, HSA, M-HSA or M-HSA+FPS-ZM1 (commercial kits) ##P < 0.01 ***P < 0.001, ###P < 0.001

Figure 3. A-C relative expressions of pro-inflammatory cytokines in the blank group, HSA, M-HSA and M-HSA+FPS-ZM1 (ELISA) ***P < 0.001, ###P < 0.001; D-E relative expressions of ROS, MDA and SOD in the blank group, HSA, M-HSA or M-HSA+FPS-ZM1 (commercial kits) ##P < 0.01 ***P < 0.001, ###P < 0.001

Figure 4. A relative expression of NO in the blank group, HSA, M-HSA or M-HSA+FPS-ZM1 (commercial kit) ***P < 0.001, ###P < 0.001; B-C Images of CE-1 and eNOS expressions in the blank group, HSA, M-HSA and M-HSA+FPS-ZM1 (commercial kit)

Figure 4. A relative expression of NO in the blank group, HSA, M-HSA or M-HSA+FPS-ZM1 (commercial kit) ***P < 0.001, ###P < 0.001; B-C Images of CE-1 and eNOS expressions in the blank group, HSA, M-HSA and M-HSA+FPS-ZM1 (commercial kit)

Figure 5. A relative expressions of Bax, Bcl-2 and C-Caspase3 in the blank group, HSA, M-HSA and M-HSA+FPS-ZM1 (WB) ***P < 0.001, ##P < 0.01, ###P < 0.001; B relative expressions of RAGE and p-p65 in the blank group, HSA, M-HSA and M-HSA+FPS-ZM1 (WB) ***P < 0.001, ##P < 0.01, ###P < 0.001; C relative expressions of ET-1, eNOS and iNOS in the blank group, HSA, M-HSA or M-HSA+FPS-ZM1 (WB) ***P < 0.001, ##P < 0.01, ###P < 0.001

Figure 5. A relative expressions of Bax, Bcl-2 and C-Caspase3 in the blank group, HSA, M-HSA and M-HSA+FPS-ZM1 (WB) ***P < 0.001, ##P < 0.01, ###P < 0.001; B relative expressions of RAGE and p-p65 in the blank group, HSA, M-HSA and M-HSA+FPS-ZM1 (WB) ***P < 0.001, ##P < 0.01, ###P < 0.001; C relative expressions of ET-1, eNOS and iNOS in the blank group, HSA, M-HSA or M-HSA+FPS-ZM1 (WB) ***P < 0.001, ##P < 0.01, ###P < 0.001