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Research Paper

Characteristics of Angiotensin I-converting enzyme 2, type II transmembrane serine protease 2 and 4 in tree shrew indicate it as a potential animal model for SARS-CoV-2 infection

, , , , , , & show all
Pages 2836-2850 | Received 25 Mar 2021, Accepted 03 Jun 2021, Published online: 06 Jul 2021

Figures & data

Table 1. The primers used for Real-Time qPCR in this study

Table 2. Homologous matching rates of tree shrews CDS and protein sequences with other species

Figure 1. Comparison of the ACE2 amino-acid sequences of tree shrew with other species

Figure 1. Comparison of the ACE2 amino-acid sequences of tree shrew with other species

Figure 2. Phylogenetic tree of ACE2 protein sequence in different species

Figure 2. Phylogenetic tree of ACE2 protein sequence in different species

Figure 3. Tertiary structure prediction of ACE2 protein for tree shrews and human

Figure 3. Tertiary structure prediction of ACE2 protein for tree shrews and human

Figure 4. Differences of the key regions of ACE2 combined with SARS-CoV between tree shrew and human

Figure 4. Differences of the key regions of ACE2 combined with SARS-CoV between tree shrew and human

Figure 5. Differences of the key regions of ACE2 combined with SARS-CoV2 between tree shrew and human

Figure 5. Differences of the key regions of ACE2 combined with SARS-CoV2 between tree shrew and human

Figure 6. Expression of ACE2 gene in different tissues of tree shrew

Ts-2d-m: tree shrew aged two days for male; ts-2d-f: tree shrew aged two days for female; ts-3y-m: tree shrew aged three years for male; ts-3y-f: tree shrew aged three years for female; ts-7y-m: tree shrew aged seven years for male; ts-7y-f: tree shrew aged seven years for female.
Figure 6. Expression of ACE2 gene in different tissues of tree shrew

Figure 7. Expression of TMPRSS2 and TMPRSS4 gene in different tissues of tree shrew

Ts-2d-m: tree shrew aged two days for male; ts-2d-f: tree shrew aged two days for female; ts-3y-m: tree shrew aged three years for male; ts-3y-f: tree shrew aged three years for female; ts-7y-m: tree shrew aged seven years for male; ts-7y-f: tree shrew aged seven years for female.
Figure 7. Expression of TMPRSS2 and TMPRSS4 gene in different tissues of tree shrew

Figure 8. Western Blot expression levels of the Tree shrew ACE2, TMPRSS2 and TMPRSS4 proteins in different tissues. (a) WB result of ACE2 in different tissues of tree shrew; (b) WB result of TMPRSS2 in different tissues of tree shrew; (c) WB result of TMPRSS4 in different tissues of tree shrew. Each experiment had three repetitions

Figure 8. Western Blot expression levels of the Tree shrew ACE2, TMPRSS2 and TMPRSS4 proteins in different tissues. (a) WB result of ACE2 in different tissues of tree shrew; (b) WB result of TMPRSS2 in different tissues of tree shrew; (c) WB result of TMPRSS4 in different tissues of tree shrew. Each experiment had three repetitions

Data availability statement

All the involved data had been included in the present manuscript, and the original raw data could be obtained from the corresponding author upon reasonable request.