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Bioengineered Volume 12, 2021 - Issue 1
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Research Paper

Dynamic changes of plasma extracellular vesicle long RNAs during perioperative period of colorectal cancer

Qing Huaa Department of Anesthesiology, Fudan University Shanghai Cancer Center, Shanghai, China;b Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China;c Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai, ChinaView further author information
,
Wenhao Xub Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China;d Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, ChinaView further author information
,
Xuefang Shena Department of Anesthesiology, Fudan University Shanghai Cancer Center, Shanghai, China;b Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, ChinaView further author information
,
Xi Tianb Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China;d Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, ChinaView further author information
,
Hailiang Zhangb Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China;d Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, ChinaCorrespondence[email protected]
View further author information
,
Yan Lie Fudan University, Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University, Shanghai, ChinaCorrespondence[email protected]
View further author information
&
Pingbo Xua Department of Anesthesiology, Fudan University Shanghai Cancer Center, Shanghai, China;b Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, ChinaCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0001-8110-1011View further author information
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Pages 3699-3710 | Received 04 Mar 2021, Accepted 31 May 2021, Published online: 16 Jul 2021

Figures & data

Figure 1. Human blood EVs confirmation. EVs were isolated and purified from plasma using membrane affinity spin columns. (a) Electron microscopy image of isolated vesicles. (b) Size distribution measurements of isolated vesicles. (c) Western blots of calnexin, which can be detected in PBMCs, but not in isolated vesicles, was used as a control. EV markers TSG101 and CD63 in isolated vesicles were detected in EVs, but not in PBMCs

Figure 1. Human blood EVs confirmation. EVs were isolated and purified from plasma using membrane affinity spin columns. (a) Electron microscopy image of isolated vesicles. (b) Size distribution measurements of isolated vesicles. (c) Western blots of calnexin, which can be detected in PBMCs, but not in isolated vesicles, was used as a control. EV markers TSG101 and CD63 in isolated vesicles were detected in EVs, but not in PBMCs

Figure 2. Comprehensive mRNAs in extracellular vesicles and functional annotations before and after surgery. (a-c) Significant DEGs in extracellular vesicles were screened and identified using the ‘Limma’ R package between samples before surgery and after extubation (Fig. A), or 1 day after surgery (Fig. B), or 3 days after surgery (Fig. C). (d) A total of 11 common DEGs were obtained in extracellular vesicles before and different time points after surgery using a Venn diagram

Figure 2. Comprehensive mRNAs in extracellular vesicles and functional annotations before and after surgery. (a-c) Significant DEGs in extracellular vesicles were screened and identified using the ‘Limma’ R package between samples before surgery and after extubation (Fig. A), or 1 day after surgery (Fig. B), or 3 days after surgery (Fig. C). (d) A total of 11 common DEGs were obtained in extracellular vesicles before and different time points after surgery using a Venn diagram

Figure 3. The functional annotations of mRNAs in EVs. (a) Biological processes, cellular components, and molecular function analysis from GO items of 11 hub genes were evaluated. (b) The protein–protein interaction network was used, showing direct interactions and potential associations between proteins. (c) The 11 hub genes most significantly involved in changed GO functions. (d) Significantly altered KEGG and Reactome pathways were predicted

Figure 3. The functional annotations of mRNAs in EVs. (a) Biological processes, cellular components, and molecular function analysis from GO items of 11 hub genes were evaluated. (b) The protein–protein interaction network was used, showing direct interactions and potential associations between proteins. (c) The 11 hub genes most significantly involved in changed GO functions. (d) Significantly altered KEGG and Reactome pathways were predicted

Figure 4. Comprehensive lncRNAs in EVs before and after surgery. (a-c) Significant lncRNAs in EVs were also screened and identified using the ‘Limma’ R package between samples before surgery and after extubation (Fig. A), or 1 day after surgery (Fig. B), or 3 days after surgery (Fig. C). (d) A total of 3 common DEGs were obtained in EVs before and different time points after surgery using a Venn diagram

Figure 4. Comprehensive lncRNAs in EVs before and after surgery. (a-c) Significant lncRNAs in EVs were also screened and identified using the ‘Limma’ R package between samples before surgery and after extubation (Fig. A), or 1 day after surgery (Fig. B), or 3 days after surgery (Fig. C). (d) A total of 3 common DEGs were obtained in EVs before and different time points after surgery using a Venn diagram

Figure 5. Cell source analysis of EVs. (a) The correlation of immune and stroma cell expression derived from EVs. (b) Dynamic changes of cells derived from EVs before and after surgery. (c) Comparison of immune cells producing EVs before and after surgery. * p < 0.05, ** p < 0.01, *** p < 0.01 vs before surgery

Figure 5. Cell source analysis of EVs. (a) The correlation of immune and stroma cell expression derived from EVs. (b) Dynamic changes of cells derived from EVs before and after surgery. (c) Comparison of immune cells producing EVs before and after surgery. * p < 0.05, ** p < 0.01, *** p < 0.01 vs before surgery

Significant DELRs between T0 and T1

Significant DELRs between T0 and T2

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Significant DEGs between T0 and T1

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Table

Significant DEGs between T0 and T2

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Table

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Significant DEGs between T0 and T3

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