Long noncoding RNA SNHG20 regulates cell migration, invasion, and proliferation via the microRNA-19b-3p/RAB14 axis in oral squamous cell carcinoma

Figures & data

Figure 1. LncRNA SNHG20 was overexpressed in OSCC tissues and CAL27 and SCC25 cells

RT-qPCR analysis of lncRNA SNHG20 in OSCC tissues (A) and cells (B). Data are presented as mean ± SD. * p < 0.05; ** p < 0.01.

Figure 2. The knockdown of lncRNA SNHG20 decreased the proliferation, migration, and invasion of SCC25 and CAL27 cells

(A) RT-qPCR analysis of lncRNA SNHG20 in OSCC cells transfected with si-SNHG20 and si-NC. (B) An MTT assay was used to explore the effect of si-SNHG20 on the proliferation of CAL27 and SCC25 cell lines. The number of migratory (C) and invasive (D) cells was determined using Transwell assays. Data are presented as mean ± SD. ** p < 0.01.

Figure 3. LncRNA SNHG20 regulates the OSCC cell functions by sponging miR-19b-3p

(A) The predicted lncRNA SNHG20 binding site in the miR-19b-3p 3'-UTR. (B) The relative luciferase activity of cells after co-transfection with wild type (WT) or mutant (Mut) lncRNA SNHG20 3'-UTR reporter genes and miR-19b-3p mimics. (C) The RNA pull-down assay further confirmed that SNHG20 could bind to miR-19b-3p. (D) RT-qPCR analysis was used to explore the effect of si-SNHG20 on the miR-19b-3p level of CAL27 and SCC25 cells. (E) RT-qPCR analysis of miR-19b-3p in OSCC tissues. Data are presented as mean ± SD. * p < 0.05; ** p < 0.01.

Figure 4. The knockdown of miR-19b-3p reversed the effect of si-SNHG20 on the proliferation, migration, and invasion of SCC25 and CAL27 cells

(A) RT-qPCR analysis of miR-19b-3p in SCC25 and CAL27 cells transfected with si-SNHG20 and miR-19b-3p inhibitor. (B) An MTT assay was used to explore the effect of si-SNHG20 and miR-19b-3p inhibitor on the proliferation of CAL27 and SCC25 cells. The number of migratory (C) and invasive (D) cells was determined using Transwell assays. Data are presented as mean ± SD. ** p < 0.01 vs. si-NC group; # p < 0.05 vs. si-SNHG20 + inhibitor NC group.

Figure 5. RAB14 is the target gene of miR-19b-3p in OSCC

(A) The predicted RAB14 binding site in the miR-19b-3p 3'-UTR. (B) Relative luciferase activity of cells after co-transfection with wild type (WT) or mutant (Mut) RAB14 3'-UTR reporter genes and miR-19b-3p mimics. (C) The RNA pull-down assay further confirmed that RAB14 could bind to miR-19b-3p. RT-qPCR (D) and western blot (E) analysis were used to explore the effect of miR-19b-3p mimic on the miR-19b-3p mRNA and protein expression in CAL27 and SCC25 cells. (F) RT-qPCR analysis of RAB14 in OSCC tissues. Data are presented as mean ± SD. * p < 0.05; ** p < 0.01.

Figure 6. Overexpression of RAB14 reversed the effect of miR-19b-3p mimic on the proliferation, migration, and invasion of SCC25 and CAL27 cells

(A) RT-qPCR analysis of RAB14 in SCC25 and CAL27 cells transfected with miR-19b-3p mimic and OE-RAB14. (B) An MTT assay was used to explore the effect of miR-19b-3p mimic and OE-RAB14 on the proliferation of CAL27 and SCC25 cells. The number of migratory (C) and invasive (D) cells was determined using Transwell assays. Data are presented as mean ± SD. ** p < 0.01 vs. mimic NC group; # p < 0.05 vs. miR-19b-3p mimic and Ad-NC group.

Data availability statement

The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.