MicroRNA 200a inhibits liver fibrosis of schistosoma

Figures & data

Table 1. Primer information

Primer name

Sequence

Hum-miR-200a Hum-TGF-β2 Mmu-α-SMA Mmu-Colla I Mmu-TGF-β2 Mmu-IFN-γ Mmu-IL-4 U6

Forward(5′–3′) CGTGGAGTCGGCAATTCAGTTGAGACATCGTT Reverse(5′–3′) ACACTCCAGCTGGGTAACACTGTCTGGTAA Forward(5′–3′) TTGTGCTCCAGACAGTCCCAG Reverse(5′–3′) CAATCCGTTGTTCAGGCACTCT Forward(5′–3′) TCCCTGGAGAAGAGCTACGAA Reverse(5′–3′) ATAGGTGGTTTCGTGGATGCC Forward(5′–3′) TGACTGGAAGAGCGGAGAGT Reverse(5′–3′) AGACGGCTGAGTAGGGAACA Forward(5′–3′) TGGTGTTGTACAGGCTGAGG Reverse(5′–3′) CCACAAAGACAGGAACCTGG Forward(5′–3′) GCTTTGCAGCTCTTCCTCAT Reverse(5′–3′) TCTTCCACATCTATGCCACTTG Forward(5′–3′) CGTCTGTAGGGCTTCCAAGG Reverse(5′–3′) AGGCTACGAAAAAGCCCGAA Forward(5′–3′) CTCGCTTCGGCAGCACA

Figure 1. qRT-PCR identification of miR-200a (a), TGF-β2 and α-SMA (b), and Colla I (c) on expression in liver tissues of mice infected with S. japonicum **: P < 0.01 vs 4 w *: P < 0.05 vs 4 w.

Figure 2. qRT-PCR identification of miR-200a (a), TGF-β2 (b), α-SMA (c), CollaI (d) and serum ALT (e) on expression in liver tissues of mice infected with S. japonicum. **: P < 0.01 vs normal control *: P < 0.05 vs normal control #: P < 0.05 vs schistosomiasis and Lenti-NC

Figure 3. Liver tissue of the mice infected with S. japonicum by HE staining

Figure 4. Liver tissue of the mice infected with S. japonicum by Masson staining

Figure 5. The effect of miR-200a on mRNA of IL-4 (a) and IFN-γ (b) by qRT-PCR, and serum level of IL-4 (c) and IFN-γ (d) by ELISA. **: P < 0.01 vs normal control *: P < 0.05 vs normal control #: P < 0.05 vs schistosomiasis and Lenti-NC