MicroRNA-301b-3p facilitates cell proliferation and migration in colorectal cancer by targeting HOXB1

Figures & data

Figure 1. The detection of miR-301b-3p suggested that it is upregulated in colorectal cancer. (a) Expression of miR-301b-3p in CRC tissue. (b) K–M plotter analyze the overall survival of miR-301b-3p. (c) Expression of miR-301b-3p in cell lines. *P< 0.05 and **P < 0.01

Figure 2. MiR-301b-3p mimics promoted cell growth and invasion in CRC in vitro. (a) Assessment of miR-301b-3p mimics transfection in DLD-1 and HT-29. (b,c) CCK-8 analysis with miR-301b-3p mimics or NC in DLD-1 and HT-29. (d) Colony formation test for cell growth rate with miR-301b-3p mimics in DLD-1 and HT-29. (e,f) Wound-healing and transwell assay for cell migration with miR-301b-3p mimics in DLD-1 and HT-29. *P < 0.05 and **P < 0.01

Figure 3. Targeting of HOXB1 by miR-301b-3p in CRC. (a) 3ʹ-UTR region of HOXB1 with the binding site for miR-301b-3p in WT and mut plasmids. The luciferase activity of HOXB1 WT or MUT 3ʹ-UTR with miR-301b-3p mimics in CRC. (b,c) The mRNA and protein level of HOXB1 affected with miR-301b-3p mimics in HT-29 and DLD-1. (d) The expression of HOXB1 mRNA is unregulated in adjacent of colorectal tissues. (e) The relationship of HOXB1 and miR-301b-3p in CRC. (R² = −0.55, P< 0.01). *P < 0.05 and **P < 0.01

Figure 4. HOXB1 promotes CRC growth and migration while it is knocking down. (a,b) The mRNA and protein of HOXB1 affected by si-HOXB1 siRNAs in CRC. (c) CCK-8 assay with si-HOXB1. (d) Colony formation test for cell growth with si-HOXB1 in CRC. (e,f) Transwell and wound-healing assay for cell migration with si-HOXB1 in CRC. *P < 0.05 and **P < 0.01

Figure 5. MiR-301b-3p effect rescued by the overexpression of HOXB1 in CRC. (a,b) Expression of mRNA and protein of HOXB1 in CRC under transfection of miR-301b-3p and plasmid of pcDNA-HOXB1. (c) Cell proliferation in CRC under transfection of miR-301b-3p and pcDNA-HOXB1 plasmid. (d) Colony formation analysis for cell growth under transfection of miR-301b-3p and pcDNA-HOXB1 plasmid. (e,f) Transwell and wound-healing test for CRC migration under transfection of miR-301b-3p and pcDNA-HOXB1. *P< 0.05 and **P < 0.01