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Research Paper

Effects of fluid shear stress on expression of focal adhesion kinase in MG-63 human osteoblast-like cells on different surface modification of titanium

, , , & ORCID Icon
Pages 4962-4971 | Received 21 Jun 2021, Accepted 28 Jul 2021, Published online: 10 Aug 2021

Figures & data

Figure 1. The surface characterization of different titanium. The SEM picture of PT group (a) and SLA group (b), and the mean values of the different titanium surfaces (c)

Figure 1. The surface characterization of different titanium. The SEM picture of PT group (a) and SLA group (b), and the mean values of the different titanium surfaces (c)

Figure 2. Effect of FSS on the proliferation of MG63 on different titanium implant. Cells were cultured in glass slide (control group), polished treatment titanium surface (PT group), and sandblasted/acid-etched surfaces titanium surface (SLA group). Cells were under static condition (a) and exposed to FSS (8 dynes/cm2 (b), 12 dynes/cm2 (c), 16 dynes/cm2 (d)) for the indicated times. *P < 0.05, **P < 0.01, ***P < 0.001

Figure 2. Effect of FSS on the proliferation of MG63 on different titanium implant. Cells were cultured in glass slide (control group), polished treatment titanium surface (PT group), and sandblasted/acid-etched surfaces titanium surface (SLA group). Cells were under static condition (a) and exposed to FSS (8 dynes/cm2 (b), 12 dynes/cm2 (c), 16 dynes/cm2 (d)) for the indicated times. *P < 0.05, **P < 0.01, ***P < 0.001

Figure 3. Effect of FSS on the FAK gene expression of MG63 on different titanium implant. Cells were exposed to FSS (8 dynes/cm2 (a), 12 dynes/cm2 (b), 16 dynes/cm2 (c)). *P < 0.05, **P < 0.01, ***P < 0.001

Figure 3. Effect of FSS on the FAK gene expression of MG63 on different titanium implant. Cells were exposed to FSS (8 dynes/cm2 (a), 12 dynes/cm2 (b), 16 dynes/cm2 (c)). *P < 0.05, **P < 0.01, ***P < 0.001

Figure 4. Effect of FSS on the FAK gene expression of MG63 on different titanium implant. Cells were exposed to FSS at 8 dynes/cm2 (a–c), 12 dynes/cm2 (d–f), 16 dynes/cm2 (g–i). The western blot results of FAK and P-FAK in cells exposed to FSS at 8 dynes/cm2 (a), 12 dynes/cm2 (d) and 16 dynes/cm2 (g). The proteins levels FAK were calculated in cells exposed to FSS at 8 dynes/cm2 (b), 12 dynes/cm2 (e) and 16 dynes/cm2 (h). The proteins levels P-FAK were calculated in cells exposed to FSS at 8 dynes/cm2 (c), 12 dynes/cm2 (f) and 16 dynes/cm2 (i). *P < 0.05, **P < 0.01, ***P < 0.001

Figure 4. Effect of FSS on the FAK gene expression of MG63 on different titanium implant. Cells were exposed to FSS at 8 dynes/cm2 (a–c), 12 dynes/cm2 (d–f), 16 dynes/cm2 (g–i). The western blot results of FAK and P-FAK in cells exposed to FSS at 8 dynes/cm2 (a), 12 dynes/cm2 (d) and 16 dynes/cm2 (g). The proteins levels FAK were calculated in cells exposed to FSS at 8 dynes/cm2 (b), 12 dynes/cm2 (e) and 16 dynes/cm2 (h). The proteins levels P-FAK were calculated in cells exposed to FSS at 8 dynes/cm2 (c), 12 dynes/cm2 (f) and 16 dynes/cm2 (i). *P < 0.05, **P < 0.01, ***P < 0.001

Data availability statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.