Figures & data
Figure 1. Expression of miR-195-5p and TERT in thyroid cancer cells and normal cells. (a)The TERT expression was evaluated by Immunohistochemistry, (200×). (b) The miR-195-5p and TERT mRNA expression was evaluated by qRT-PCR.(c) The TERT protein expression was evaluated by western blot. **P < 0.01 vs. CAL-62 group. Each experiment was repeated three times
Figure 2. miR-195-5p overexpression can inhibit the proliferation of thyroid cancer cell CAL-62, induce its apoptosis and inhibit its invasive ability. (a) The miR-195-5p mRNA expression was evaluated by qRT-PCR.(b) The cell viability was evaluated by MTT assay. (c) The apoptosis of cells was evaluated by flow cytometry. (d) The invasion of cells was evaluated by Transwell, (200×). (e) The Ki67, PCNA, Caspase-3 and Caspase-9 protein expressions were evaluated by western blot. **P < 0.01, ***P < 0.001 vs. Control group
Figure 3. miR-195-5p targets and regulates the expression of TERT, knocking down the expression of TERT can also inhibit the cell proliferation, induce apoptosis and inhibit invasion ability. (a) TargetScan predicts miR-195-5p and TERT binding sites. (b) Luciferase activity analysis of the targeted relationship of miR-195-5p and TERT in HEK-293 T cells. (c) The TERT protein expression was evaluated by western blot. (d) The cell viability was evaluated by CCK-8 assay. (e) The apoptosis of cells was evaluated by flow cytometry. (f) The invasion of cells was evaluated by Transwell, (200×). (g) The TERT, Ki67, PCNA, Caspase-3 and Caspase-9 protein expressions were evaluated by western blot. **P < 0.01 vs. Control group or NC mimics,#P < 0.05 vs. NC inhibitor
Figure 4. Overexpression of TERT can partially reverse the effect of overexpression of miR-195-5p on cell proliferation, apoptosis and invasion ability. (a) The cell viability was evaluated by CCK-8 assay. (b) The apoptosis of cells was evaluated by flow cytometry. (c) The invasion of cells was evaluated by Transwell, (200×). (d) The TERT, Ki67, PCNA, Caspase-3 and Caspase-9 protein expressions were evaluated by western blot. **P < 0.01 vs. NC-mimicsgroup,##P < 0.01 vs. miR-195-5p + pcDNA 3.0 group
Availability of data and material
All data are available from the corresponding authors on reasonable request.