Figures & data
Table 1. Quantitative primers
Figure 1. Gastrodin promotes the proliferation of HUVECs A: determination of cell proliferation using EdU; B: assessment of cell proliferation using MTT. * p < 0.05; ** p < 0.01. GSTD, gastrodin
Figure 2. Gastrodin promotes the migration and tube formation of HUVECs A: determination of cell migration ability using scratch test; B: assessment of the expression of MMP-2 using qRT-PCR; C: assessment of the expression of MMP-9 using qRT-PCR; D: evaluation of cell tube formation ability using tube formation test. * p < 0.05; ** p < 0.01. GSTD, gastrodin
Figure 3. Gastrodin upregulates miR-21 expressionDetermination of miR-21 expression level using qRT-PCR; * p < 0.05; ** p < 0.01 vs. control group. # p < 0.05 vs. GSTD-10 µM group. GSTD, gastrodin
Figure 4. Gastrodin affects the proliferation, migration and tube formation ability of HUVECs via miR-21 A: determination of miR-21 expression level using qRT-PCR, *** represented comparison with NC inhibitor group, and p < 0.001; B: assessment of cell proliferation using MTT; C: determination of MMP-2 expression using qRT-PCR; D: determination of MMP-9 expression using qRT-PCR; E: assessment of the migration ability using scratch test; F: assessment of the tube forming ability using tube formation test. ** p < 0.01; ## represented comparing to GSTD+NC inhibitor group, and p < 0.01. GSTD, gastrodin
Figure 5. Gastrodin activates the PI3K/Akt signaling pathway of HUVECs evaluation of the protein expression levels of PI3K, p-PI3K, Akt and p-Akt using western blot. **p < 0.01; ## represented comparing to GSTD+NC inhibitor group, and p < 0.01. GSTD, gastrodin
