Figures & data
Figure 1. Treatment Of LYC Alleviated Cardiac Dysfunction Induced By ISO. (a) A Summary Of Animal Treatments Procedure. (b) Measurements Of Echocardiography In Different Groups Of Mice. (c,d) Ejection Fraction (%) And Fractional Shortening (%) Of Heart Were Evaluated By Echocardiography. E And F, The Levels Of Specific Cardiac Injury Biomarkers (CK-MB And LDH) In Plasma. *P < 0.05 And **P < 0.01 Mean Statistic Significance
Table 1. All Primer Sequences Used In Qrt-PCR Experiment In The Present Study
Figure 2. Treatment Of LYC Ameliorated Histopathological Injury In Heart Induced By ISO. (a,–c) The Levels Of Inflammatory Cytokines Like IL-1β, IL-6 And TNF-Α Were Assessed By Assay Of ELISA. (d) The Results Of H&E Staining And Massion Staining. (e) Heart Injury Score Was Used To Assess Degree Of Cardiac Injury. (f) Fibrosis Area (%) Was Used To Evaluate Degree Of Cardiac Fibrosis. *P < 0.05 And **P < 0.01 Mean Statistic Significance
Figure 3. Treatment Of LYC Inhibited Inflammation In Heart Tissue Induced By ISO. (a) The Protein Bands Of IL-1β, IL-6 And TNF-Α Were Detected By Western Blotting. (b–d) Statistical Analyses Of Protein Levels Like IL-1β, IL-6 And TNF-Α. (e–g) Statistical Analyses Of Mrna Levels Of IL-1β, IL-6 And TNF-Α. *P < 0.05, **P < 0.01 And ***P < 0.001 Mean Statistic Significance
Figure 4. Treatment Of LYC Suppressed Cardiac Inflammation Partially By Inhibiting Activation Of The NF-Κb Signaling Pathway. (a,b) The Protein Level Of Activated NF-Κb Was Measured By Western Blotting. *P < 0.05 And **P < 0.01 Mean Statistic Significance
Figure 5. Treatment Of LYC Reduced Fibrosis In Heart Tissue Induced By ISO. (a) The Protein Bands Of Collagen I And Collagen III Were Detected By Western Blotting. (b) The Protein Bands Of Activated Smad2/3 Was Measured By Western Blotting. (c–e) Statistical Analyses Of Protein Levels Like Collagen I, Collagen III And P-Smad2/3. (f–h) Statistical Analyses Of Mrna Levels Of Collagen I, Collagen III And Fibronectin. *P < 0.05 And **P < 0.01 Mean Statistic Significance
Figure 6. Treatment Of LYC Inhibited Oxidative Stress In Heart Induced By ISO. (a,b) The Concentration Of MDA And SOD Activity In Plasma Were Assessed By Specific Kits. (c) The Result Of Assay Of DHE Staining. D, The Level Of Total ROS Was Evaluated By DHE Fluorescence Of Intensity. *P < 0.05 And **P < 0.01 Mean Statistic Significance
Figure 7. Treatment Of LYC Suppressed Apoptosis In Heart Induced By ISO. (a) The Protein Bands Of Bcl-2 And Bax Were Detected By Western Blotting. (b,c) Statistical Analyses Of Protein Levels Of Bcl-2 And Bax. *P < 0.05 And **P < 0.01 Mean Statistic Significance
