Carnitine palmitoyltransferase 1 (CPT1) alleviates oxidative stress and apoptosis of hippocampal neuron in response to beta-Amyloid peptide fragment Aβ_25-35

Figures & data

Figure 1. CPT1C expression was reduced in Aβ_25-35-induced HT22 cells. HT22 cells were treated with Aβ_25-35 (20 μM) for 6 h, 12 h, 24 h or 48 h, respectively

(a) The cell viability of Aβ_25-35-induced HT22 cells was detected using CCK-8 assay. (b) mRNA and protein expressions of CPT1C in Aβ_25-35-induced HT22 cells were measured using RT-qPCR and western blot, respectively. *P < 0.05 and ***P < 0.001 vs. Control group.

Figure 2. CPT1C overexpression attenuated cell viability and toxic injury in Aβ_25-35-induced HT22 cells. HT22 cells were transfected with Ov-CPT1C or Ov-NC for 24 h, and then treated with Aβ_25–35 for another 24 h

(a) mRNA and protein expressions of CPT1C were measured using RT-qPCR and western blot, respectively. ***P < 0.001 vs Ov-NC. (b) The cell viability of Aβ_25-35-induced HT22 cells was detected using CCK-8. ***P < 0.001 vs. Control group, ^##P < 0.01 vs Aβ_25-35 + Ov-NC. (c) The relative LDH level of Aβ_25-35-induced HT22 cells was checked using LDH assay. ***P < 0.001 vs. Control group, ^###P < 0.001 vs Aβ_25-35 + Ov-NC.

Figure 3. CPT1C overexpression attenuated oxidative stress in Aβ_25-35-induced HT22 cells. Following transfection of Ov-CPT1C or Ov-NC for 24 h, HT22 cells were treated with Aβ_25–35 for another 24 h

(a) ROS expression was detected using ROS kits. (b) MDA levels and SOD activity were measured using MDA and SOD kits, respectively. (c) The relative mRNA and protein expression of SOD1 and SOD2 in A_25-35-induced HT22 cells were measured using RT-qPCR and western blot, respectively. ***P < 0.001 vs. Control group, ^##P < 0.01 and ^###P < 0.001 vs A_25-35 + Ov-NC.

Figure 4. CPT1C overexpression decreased the apoptosis of Aβ_25-35-induced HT22 cells. Following transfection of Ov-CPT1C or Ov-NC for 24 h, HT22 cells were treated with Aβ_25–35 for another 24 h

(a) The apoptosis of Aβ_25-35-induced HT22 cells was evaluated by TUNEL. (b) The expressions of Bcl2, Bax, cleaved PARP and APAF-1 were measured by western blot. ***P < 0.001 vs. Control group, ^###P < 0.001 vs A_25-35 + Ov-NC.

Figure 5. CPT1C overexpression decreased the deposition of AD marker proteins in Aβ_25-35-induced HT22 cells. Following transfection of Ov-CPT1C or Ov-NC for 24 h, HT22 cells were treated with Aβ_25–35 for another 24 h

(a) The mRNA expressions of App, p-Tau and Bace-1 were evaluated using RT-qPCR. (b) The protein expressions of App, p-Tau and Bace-1 were evaluated using western blot. ***P < 0.001 vs. Control group, ^###P < 0.001 vs Aβ_25-35 + Ov-NC.

Figure 6. PPARα activation could increase CPT1C expression in Aβ_25-35-induced HT22 cells. HT22 cells were co-treated with gemfibrozil 100 μM or 250 μM, and Aβ_25-35 for 48 h

(a) The relative CPT1C mRNA expression in Aβ_25-35-induced HT22 cells was detected using RT-qPCR. (b) The protein expression of CPT1C expression in Aβ25-35-induced HT22 cells was detected using western blot. ***P < 0.001 vs. Control group, ^###P < 0.001 vs Aβ_25-35+ gemfibrozil 100 μM.